Tissue-Specific Transcriptome Profiling in Arabidopsis Roots.

Tissue-Specific Transcriptome Profiling in Arabidopsis Roots.
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拟南芥根的组织特异性转录组分析。

DOI:
10.1007/978-1-4939-7003-2_8
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发表时间:
2017
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
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通讯作者:
Benfey,PhilipN
Benfey,PhilipN
中科院分区:
--
文献类型:
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作者:
Sparks,ErinE;Benfey,PhilipN

文献摘要

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特定组织的时空转录组谱对于了解植物发育和对环境的反应至关重要。分离特定组织的一种方法是荧光激活细胞分选(FACS)。在本章中,我们概述了根原生质体的 FACS 分离方法,然后使用 RNA 测序进行转录组分析。
Spatiotemporal transcriptome profiles from specific tissues are critical for understanding plant development and responses to the environment. One approach to isolate specific tissues is fluorescence-activated cell sorting (FACS). In this chapter, we outline methods for the FACS isolation of root protoplasts followed by transcriptome profiling using RNA sequencing.