Tissue-Specific Transcriptome Profiling in Arabidopsis Roots.
Tissue-Specific Transcriptome Profiling in Arabidopsis Roots.
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拟南芥根的组织特异性转录组分析。
DOI:
10.1007/978-1-4939-7003-2_8
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发表时间:
2017
期刊:
影响因子:
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通讯作者:
Benfey,PhilipN
中科院分区:
文献类型:
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作者:
Sparks,ErinE;Benfey,PhilipN
Spatiotemporal transcriptome profiles from specific tissues are critical for understanding plant development and responses to the environment. One approach to isolate specific tissues is fluorescence-activated cell sorting (FACS). In this chapter, we outline methods for the FACS isolation of root protoplasts followed by transcriptome profiling using RNA sequencing.