Discovery of a novel short peptide with efficacy in accelerating the healing of skin wounds
Discovery of a novel short peptide with efficacy in accelerating the healing of skin wounds
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发现一种新型短肽，具有加速皮肤伤口愈合的功效。
DOI：
10.1016/j.phrs.2020.105296
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发表时间：
2021-01-17
影响因子：
9.3
通讯作者：
Yang, Xinwang
中科院分区：
文献类型：
--
作者：
Wang, Ying;Feng, Zhuo;Yang, Xinwang
尽管广泛努力开发有效的治疗方法，但皮肤伤口的治疗仍然是相当大的临床挑战。现有的补救措施不能充分满足当前的需求，因此发现新的促愈合剂越来越重要。在本研究中，我们从泽蛙皮肤分泌物中鉴定了一种新的短肽（命名为RL-QN 15，一级序列为“QNSYADLWCQFHYMC”），它可以加速小鼠伤口愈合。其机制研究表明，RL-QN 15可激活MAPK和Smad信号通路，选择性地调节巨噬细胞分泌细胞因子。这导致皮肤细胞的增殖和迁移以及伤口中TGF-β 1和TGF-β 3的动态调节，这加速了再上皮化和肉芽组织形成，从而促进了皮肤再生。此外，RL-QN 15对慢性伤口、皮肤纤维化和口腔溃疡显示出显著的治疗效力。我们的研究结果突出了青蛙皮肤分泌物作为具有愈合活性的生物活性肽的潜在宝库。在本研究中鉴定的新型肽（RL-QN 15）显示出作为新型促愈合剂开发的候选物的相当大的能力。
Despite extensive efforts to develop efficacious therapeutic approaches, the treatment of skin wounds remains a considerable clinical challenge. Existing remedies cannot sufficiently meet current needs, so the discovery of novel pro-healing agents is of growing importance. In the current research, we identified a novel short peptide (named RL-QN15, primary sequence 'QNSYADLWCQFHYMC') from Rana limnocharis skin secretions, which accelerated wound healing in mice. Exploration of the underlying mechanisms showed that RL-QN15 activated the MAPK and Smad signaling pathways, and selectively modulated the secretion of cytokines from macrophages. This resulted in the proliferation and migration of skin cells and dynamic regulation of TGF-beta 1 and TGF-beta 3 in wounds, which accelerated re-epithelialization and granulation tissue formation and thus skin regeneration. Moreover, RL-QN15 showed significant therapeutic potency against chronic wounds, skin fibrosis, and oral ulcers. Our results highlight frog skin secretions as a potential treasure trove of bioactive peptides with healing activity. The novel peptide (RL-QN15) identified in this research shows considerable capacity as a candidate for the development of novel pro-healing agents.