A Kainate Receptor Linked to Nitric Oxide Synthesis from Arginine
A Kainate Receptor Linked to Nitric Oxide Synthesis from Arginine
复制标题
与精氨酸合成一氧化氮相关的红藻氨酸受体
DOI：
10.1111/j.1471-4159.1989.tb09266.x
复制
发表时间：
1989
影响因子：
4.7
通讯作者：
M. Anderton
中科院分区：
文献类型：
--
作者：
J. Garthwaite;E. Southam;M. Anderton
摘要：在年轻大鼠小脑切片中，谷氨酸类似物红藻氨酸诱导大量环GMP积聚，这可被非N-甲基-D-天冬氨酸拮抗剂抑制。使君子酸和a-氨基-3-羟基-5-甲基-4-异恶唑丙酸酯仅引起小的环GMP反应，并抑制红藻氨酸的作用。当在小脑细胞悬浮液中进行测试时，谷氨酸也是对红藻氨酸的环GMP反应的有效拮抗剂。超氧化物歧化酶增强了分离细胞的反应，而血红蛋白和亚甲蓝则具有抑制作用。切片中的反应是Ca 2+依赖性的，可被精氨酸增强，并被l-NG-单甲基精氨酸抑制，其抑制方式可以被额外的精氨酸逆转。它的结论是，红藻氨酸受体的刺激导致激活的酶，从精氨酸合成一氧化氮和激活的可溶性鸟苷酸环化酶的释放的一氧化氮帐户的环GMP生成。
Abstract: In slices of young rat cerebellum, the glutamate analogue kainate induced a large accumulation of cyclic GMP, which was inhibited by non‐TV‐methyl‐D‐aspartate antagonists. Quisqualate and a‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazoIepropionate evoked only small cyclic GMP responses and inhibited the effect of kainate. When tested in cerebellar cell suspensions, glutamate was also a potent antagonist of the cyclic GMP response to kainate. Superoxide dismutase enhanced the response in the isolated cells, whereas haemoglobin and methylene blue were inhibitory. The response in slices was Ca2+ dependent, augmented by arginine, and inhibited by l‐NG‐mono‐methylarginine in a manner that could be reversed by additional arginine. It is concluded that stimulation of kainate receptors leads to activation of the enzyme that synthesises nitric oxide from arginine and that activation of soluble guanylate cyclase by the released nitric oxide accounts for the cyclic GMP generation.