Multiple serotypes of the moderate thermophile Thiobacillus caldus, a limitation of immunological assays for biomining microorganisms
Multiple serotypes of the moderate thermophile Thiobacillus caldus, a limitation of immunological assays for biomining microorganisms
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DOI：
10.1128/aem.62.11.4243-4246.1996
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发表时间：
1996-11-01
影响因子：
4.4
通讯作者：
Lindstrom, EB
中科院分区：
文献类型：
--
作者：
Hallberg, KB;Lindstrom, EB
系统发育和表型分析表明，来自澳大利亚的中度嗜热分离株 C-SH12 属于硫杆菌 (Thiobacillus caldus)。针对 T. caldus KU 的全细胞产生的抗血清可识别 T. caldus 三种菌株 KU、BC13 和 C-SH12 的细胞裂解物所共有的蛋白质抗原，但不识别分离株 C-SH12 的全细胞。发现菌株C-SH12和其他两种T. caldus菌株的脂多糖（LPS）存在差异，并且抗KU抗血清不能识别菌株C-SH12的LPS。这些数据表明该 T. caldus 分离株属于与菌株 KU 和 BC13 不同的血清型。
Phylogenetic and phenotypic analysis indicates that a moderately thermophilic isolate, C-SH12, from Australia belongs to the species Thiobacillus caldus. Antiserum generated against whole cells of T. caldus KU recognized protein antigens common to cell lysates of the three T. caldus strains KU, BC13, and C-SH12 but did not recognize whole cells of isolate C-SH12. Differences in the lipopolysaccharide (LPS) of strain C-SH12 and those of the other two T. caldus strains were found, and the anti-KU antiserum did not recognize the LPS from strain C-SH12. These data indicate that this T. caldus isolate belongs to a serotype different from that of strains KU and BC13.