Impact of orally administered microcapsules on gastrointestinal microbial flora: In-vitro investigation using computer controlled dynamic human gastrointestinal model
Impact of orally administered microcapsules on gastrointestinal microbial flora: In-vitro investigation using computer controlled dynamic human gastrointestinal model
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DOI：
10.1080/10731190701460226
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Prakash, Satya
中科院分区：
文献类型：
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作者：
Afkhami, Fatemeh;Ouyang, Wei;Prakash, Satya
口服人造细胞微胶囊已被提议用于各种使用生物活性材料的治疗程序。最近，我们用海藻酸盐、聚l -赖氨酸、果胶、聚l -赖氨酸和海藻酸盐设计了新型的APPPA微胶囊，这些微胶囊具有优越的口服给药特性。本文采用计算机控制的动态胃肠模型，研究了APPPA微胶囊对胃肠菌群健康的影响。分析了APPPA微胶囊对胃肠道菌群、总厌氧菌、总需氧菌、大肠杆菌、乳杆菌和葡萄球菌的影响。此外，还研究了微胶囊对胃肠道微生物胞外酶活性的影响。结果表明，APPPA微胶囊的使用改变了微生物菌群和酶的活性。差异最大的是上升结肠的微生物活动。本研究对未来微胶囊设计具有重要意义。然而，还需要进一步的体内研究。
Oral administration of artificial cell microcapsules has been proposed for various therapy procedures using biologically active materials. Recently we have designed novel APPPA microcapsules using alginate, poly-L-lysine, pectin, poly-L-lysine and alginate that have shown superior oral delivery features. This article investigates, in-vitro using a computer controlled dynamic gastrointestinal (GI) model, effects of APPPA microcapsules on health of gastrointestinal (GI) microbial flora. The impact of APPPA microcapsules on GI bacterial population, total anaerobes, total aerobes, Escherichia coli, Lactobacillus sp. and Staphylococcus sp. has been analyzed. In addition, the effects of microcapsules on GI microbial extracellular enzymatic activities have been investigated. Result shows the altered activities of microbial flora and enzymes due to the use of APPPA microcapsule. The most disparity is observed in the colon ascendans microbial activities. This study would have significant impact on future microcapsule design. However, further in-vivo studies are required.