Quality evaluation of Yin Chen Hao Tang extract based on fingerprint chromatogram and simultaneous determination of five bioactive constituents
Quality evaluation of Yin Chen Hao Tang extract based on fingerprint chromatogram and simultaneous determination of five bioactive constituents
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基于指纹图谱和五种活性成分同时测定的茵陈蒿汤提取物质量评价
DOI：
10.1002/jssc.200700376
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发表时间：
2008-01-01
影响因子：
3.1
通讯作者：
Bi, Kaishun
中科院分区：
文献类型：
--
作者：
Wang, Xijun;Lv, Haitao;Bi, Kaishun
描述了一种基于高效液相色谱法与光电二极管阵列检测器 (HPLC-UV) 相结合的完全验证的方法，用于评估和控制茵陈蒿汤提取物 (YCHTE) 的质量。首先建立了YCHTE的HPLC-UV指纹图谱，初步阐明了YCHTE中化学成分的含量和色谱轨迹。其次，首次基于指纹图谱同时测定了YCHTE中5种主要生物活性成分，进一步定量控制了YCHTE的质量。所开发的方法用于分析 12 批由不同产地的草药组成的 YCHTE 样品，显示出可接受的线性，日内（RSD
A completely validated method based on HPLC coupled with photodiode array detector (HPLC-UV) was described for evaluating and controlling quality of Yin Chen Hao Tang extract (YCHTE). First, HPLC-UV fingerprint chromatogram of YCHTE was established for preliminarily elucidating amount and chromatographic trajectory of chemical constituents in YCHTE. Second, for the first time, five mainly bioactive constituents in YCHTE were simultaneously determined based on fingerprint chromatogram for furthermore controlling the quality of YCHTE quantitatively. The developed method was applied to analyze 12 batches of YCHTE samples which consisted of herbal drugs from different places of production, showed acceptable linearity, intraday (RSD