PoxCbh, a novel CENPB-type HTH domain protein, regulates cellulase and xylanase gene expression in Penicillium oxalicum
PoxCbh, a novel CENPB-type HTH domain protein, regulates cellulase and xylanase gene expression in Penicillium oxalicum
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PoxCbh 是一种新型 CENPB-型 HTH 结构域蛋白，可调节草酸青霉中的纤维素酶和木聚糖酶基因表达
DOI：
10.1111/mmi.14696
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发表时间：
2021-03-13
影响因子：
3.6
通讯作者：
Feng, Jia-Xun
中科院分区：
文献类型：
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作者：
Li, Cheng-Xi;Liao, Lu-Sheng;Feng, Jia-Xun
丝状真菌Penicillium puericum中的纤维素酶和木聚糖酶基因表达都需要转录因子PoxCxrA，由于存在整合的纤维素酶和木聚糖酶系统，因此在生物技术工业中具有潜在的应用前景。然而，真菌中与PoxCxrA调控特异性相关的纤维素酶和木聚糖酶基因表达的调控机制知之甚少。在这项研究中，新的调节PoxCbh（POX 06865），含有一个着丝粒蛋白B-型螺旋-转角-螺旋结构域，通过筛选的PoxCxrA调节子在Avicel诱导和遗传分析确定。突变株poxCbh的纤维素酶和木聚糖酶产量均显著下降，降幅为28.4%~ 59.8%。此外，发现PoxCbh直接调控重要的纤维素酶和木聚糖酶基因的表达，以及已知的调控基因PoxNsdD和POX 02484，其表达直接由PoxCxrA控制。鉴定了纤维二糖水解酶1基因cbh 1启动子区的PoxCbh结合DNA序列。这些结果扩展了我们对真菌中着丝粒蛋白B样蛋白、纤维素酶和木聚糖酶基因表达的调控网络以及调控机制的理解。
The essential transcription factor PoxCxrA is required for cellulase and xylanase gene expression in the filamentous fungus Penicillium oxalicum that is potentially applied in biotechnological industry as a result of the existence of the integrated cellulolytic and xylolytic system. However, the regulatory mechanism of cellulase and xylanase gene expression specifically associated with PoxCxrA regulation in fungi is poorly understood. In this study, the novel regulator PoxCbh (POX06865), containing a centromere protein B‐type helix‐turn‐helix domain, was identified through screening for the PoxCxrA regulon under Avicel induction and genetic analysis. The mutant ∆PoxCbh showed significant reduction in cellulase and xylanase production, ranging from 28.4% to 59.8%. Furthermore, PoxCbh was found to directly regulate the expression of important cellulase and xylanase genes, as well as the known regulatory genes PoxNsdD and POX02484, and its expression was directly controlled by PoxCxrA. The PoxCbh‐binding DNA sequence in the promoter region of the cellobiohydrolase 1 gene cbh1 was identified. These results expand our understanding of the diverse roles of centromere protein B‐like protein, the regulatory network of cellulase and xylanase gene expression, and regulatory mechanisms in fungi.