Molecular heterogeneity in adjacent cells in triple-negative breast cancer
Molecular heterogeneity in adjacent cells in triple-negative breast cancer
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DOI：
10.2147/bctt.5.67041
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发表时间：
2015-01-01
影响因子：
2.6
通讯作者：
Frenkel, Eugene P.
中科院分区：
文献类型：
--
作者：
Huebschman, Michael L.;Lane, Nancy L.;Frenkel, Eugene P.
目的：本研究探讨了三阴性乳腺癌患者个体细胞的分子状态，并探讨了这些肿瘤的分子鉴定和表征，以考虑开发潜在的靶向治疗方法。患者和方法：高光谱免疫细胞通过细胞分析应用于从乳腺癌患者的新鲜肿瘤获得的触摸印迹涂片。逐个细胞分析证实了肿瘤标志物的显著的肿瘤内分子异质性，与聚合酶链反应标志物报告存在差异。单个细胞异质性在相邻细胞中被识别，在每个单细胞中用10个分子标记物的组进行检查，并且包括一些被认为表达“干细胞”特征的标记物。此外，异质性并不涉及无论是原发性肿瘤的大小或阶段或从cancer.Conclusion网站：有一个非常显着的分子异质性时，“相邻细胞”在三阴性乳腺癌进行检查，从而使成功的有针对性的方法不太可能。此外，认为这些变化将为肿瘤休眠提供答案是不合理的。
Purpose: This study interrogates the molecular status of individual cells in patients with triple-negative breast cancers and explores the molecular identification and characterization of these tumors to consider the exploitation of a potential-targeted therapeutic approach.Patients and methods: Hyperspectral immunologic cell by cell analysis was applied to touch imprint smears obtained from fresh tumors of breast cancer patients.Results: Cell by cell analysis confirms significant intratumoral molecular heterogeneity in cancer markers with differences from polymerase chain reaction marker reporting. The individual cell heterogeneity was recognized in adjacent cells examined with panels of ten molecular markers in each single cell and included some markers that are considered to express "stem-cell" character. In addition, heterogeneity did not relate either to the size or stage of the primary tumor or to the site from within the cancer.Conclusion: There is a very significant molecular heterogeneity when "adjacent cells" are examined in triple-negative breast cancer, thereby making a successful targeted approach unlikely. In addition, it is not reasonable to consider that these changes will provide an answer to tumor dormancy.