METABOLISM OF RADIOLABELED ENERGY-YIELDING SUBSTRATES BY RAT SERTOLI CELLS
METABOLISM OF RADIOLABELED ENERGY-YIELDING SUBSTRATES BY RAT SERTOLI CELLS
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DOI：
10.1530/jrf.0.0770109
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发表时间：
1986-05-01
期刊：
影响因子：
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通讯作者：
VANDERMOLEN, HJ
中科院分区：
文献类型：
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作者：
GROOTEGOED, JA;OONK, RB;VANDERMOLEN, HJ
研究了未成熟大鼠支持细胞体外对3H-或14c标记的葡萄糖、丙酮酸、谷氨酰胺和亮氨酸的代谢率。葡萄糖的总体利用率远远超过了通过柠檬酸循环和通过戊糖磷酸途径氧化分支的葡萄糖代谢的丙酮酸（来源于葡萄糖）的氧化速率。这种葡萄糖代谢模式在FSH刺激葡萄糖代谢后没有明显改变。外源性丙酮酸的氧化速率表明，支持细胞葡萄糖代谢的能量产量可能取决于细胞外丙酮酸和乳酸的浓度。没有证据表明高速率的有氧糖酵解对支持细胞是至关重要的。在含有葡萄糖和所有氨基酸的培养基中，14c标记的谷氨酰胺和亮氨酸以相当快的速度转化为14CO2。据计算，除了葡萄糖和脂肪酸外，谷氨酰胺和亮氨酸的氧化可以产生支持细胞所需的大部分能量。
The rates of metabolism in vitro of 3H- or 14C-labeled glucose, pyruvate, glutamine and leucine by Sertoli cells from immature rats were examined. The overall rate of glucose utilization exceeded by far the rates of oxidation of pyruvate (derived from glucose) via the citric acid cycle and glucose metabolism via the oxidative branch of the pentose phosphate pathway. This pattern of glucose metabolism was not markedly altered after stimulation of glucose metabolism by FSH. The rate of oxidation of exogenous pyruvate indicated that the energy yield from glucose metabolism by Sertoli cells could be dependent on the extracellular concentrations of pyruvate and lactate. There is no evidence that a high rate of aerobic glycolysis is of vital importance for Sertoli cells. In medium containing glucose and all amino acids, 14C-labelled glutamine and leucine were converted to 14CO2 at considerable rates. It was calculated that the oxidation of glutamine and leucine in addition to glucose and fatty acids can yield much of the required energy of Sertoli cells.