Association between ethanol and sucrose intake in the laboratory mouse: Exploration via congenic strains and conditioned taste aversion
Association between ethanol and sucrose intake in the laboratory mouse: Exploration via congenic strains and conditioned taste aversion
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DOI：
10.1097/00000374-200003000-00001
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发表时间：
2000-03-01
影响因子：
3.2
通讯作者：
McClearn, GE
中科院分区：
文献类型：
--
作者：
Blizard, DA;McClearn, GE
背景资料：大量文献表明，在大鼠和小鼠的近交系中，“甜”溶液和乙醇的摄入量呈正相关，但有人推测这种相关性是偶然的，并且对潜在机制没有一致意见。方法和结果：我们评估了通过回交有利于BXD RI-1基因蔗糖摄入的等位基因所产生的同类小鼠中蔗糖和乙醇摄入量之间的相关性。5株转化为DBA/2 J。此外，为了更具体地探讨两种溶液摄入量之间的相互关系，我们研究了C57 BL/6 J小鼠从蔗糖到乙醇的厌恶泛化。在同类小鼠中，发现6小时蔗糖摄入量与96小时2瓶试验中呈现的10%乙醇偏好之间存在统计学显著的积差相关r = 0.36（p < 0.02）。此外，C57 BL/6 J雄性小鼠适应于避免0.2 M蔗糖溶液，将其厌恶推广到相同的2瓶测试中呈现的10%乙醇溶液，饮用42.1 +/- 9.38%。与对照组平均值69.86 +/-8.84%相比，他们从乙醇管中摄入的总液体量（平均值+/- SE）。同类小鼠中蔗糖和乙醇摄入量之间的正相关性提供了强有力的证据，证明先前证明的这些溶液摄入量之间的遗传相关性不是不相关等位基因偶然固定的结果，并提供了暗示性证据，至少在B6/D2谱系中，两种溶液摄入量之间的遗传关联反映了相同基因的紧密连锁或多效性效应。在C57 BL/6 J近交系小鼠品系中，对蔗糖的条件性味觉厌恶一般化为对乙醇的条件性味觉厌恶，这一证明是远交系大鼠中类似观察结果的延伸，并特别证明了两种溶液的摄入受一些相同的生理或神经过程控制，因此与遗传相关性的多效性解释一致。
Background: A substantial body of literature indicates that intakes of "sweet" solutions and ethanol are positively correlated across inbred strains of rats and mice but there has been speculation that the correlation is fortuitous and there is no agreement on the underlying mechanism.Methods and Results: We assessed the correlation between intake of sucrose and ethanol in congenic mice created by backcrossing alleles favoring sucrose intake from the BXD RI-5 strain into DBA/2J. In addition, to probe more specifically the interrelationship between intake of the two solutions, we examined aversion generalization from sucrose to ethanol in C57BL/6J mice. Among the congenic mice, a statistically significant product-moment correlation of r = 0.36 (p < 0.02) was found between 6-hr intake of sucrose corrected for differences in baseline water intake and preference for 10% ethanol presented in a 96-hr 2-bottle test. Furthermore, C57BL/6J male mice conditioned to avoid a 0.2 M sucrose solution generalized their aversion to a 10% ethanol solution presented in the same 2-bottle test, drinking 42.1 +/- 9.38% (mean +/- SE) of their total fluid intake from the ethanol tube, compared with the control group mean of 69.86 +/- 8.84%.Conclusions: The positive association between intake of sucrose and ethanol in congenic mice provides strong evidence that the previously demonstrated genetic correlation between intake of these solutions is not the result of fortuitous fixation of unrelated alleles and provides suggestive evidence that, at least in the B6/D2 lineage, the genetic association between intakes of the two solutions reflects close linkage or the pleiotropic effects of the same genes. The demonstration that a conditioned taste aversion to sucrose generalized to ethanol in the C57BL/6J inbred mouse strain is an extension of similar observations in outbred rats and specifically demonstrates that intake of the two solutions is controlled by some of the same physiologic or neurological processes and thus is consistent with the pleiotropic interpretation of the genetic correlation.