Downregulation of KLF4 and the Bcl-2/Bax ratio in advanced epithelial ovarian cancer
Downregulation of KLF4 and the Bcl-2/Bax ratio in advanced epithelial ovarian cancer
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DOI：
10.3892/ol.2012.834
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发表时间：
2012-11-01
期刊：
影响因子：
2.9
通讯作者：
Roh, Jaesook
中科院分区：
文献类型：
--
作者：
Yoon, Ok;Roh, Jaesook
Kruppel样因子4（KLF 4）是细胞分化和增殖的关键转录调节因子，并且已报道在许多人类恶性肿瘤中KLF 4的表达改变。在本研究中，我们研究了KLF 4的表达及其在晚期上皮性卵巢癌（FOC）细胞增殖中的作用。我们比较了晚期卵巢上皮性癌和正常对照卵巢中KLF 4、Bcl-2和Bax的转录水平。将KLF 4基因导入卵巢癌细胞，采用实时荧光定量PCR和MTT法分别检测Bcl-2和Bax的转录水平和细胞增殖情况。卵巢KLF 4表达和Bcl-2/Bax比值在大多数晚期EOC中下调。此外，KLF 4在卵巢癌细胞中的过表达增加了Bcl-2/Bax比率。MTT分析显示KLF 4过表达对卵巢癌细胞的增殖无影响。在卵巢癌中经常观察到KLF 4的失活，并且在卵巢癌中KLF 4的表达减少可能导致Bcl-2/Bax比率的降低。后者在预测癌症分级方面具有作用，尽管其在卵巢癌发生中的确切作用需要澄清。
Kruppel-like factor 4 (KLF4) is a key transcriptional regulator of cell differentiation and proliferation and an altered expression of KLF4 has been reported in a number of human malignancies. In the present study, we investigated KLF4 expression and its role in cell proliferation in advanced epithelial ovarian cancer (FOC). We compared KLF4, Bcl-2 and Bax transcript levels in ovaries isolated from advanced EOC and normal control ovaries. In addition, the KLF4 gene was transduced into ovarian cancer cells and transcript levels of Bcl-2 and Bax and cell proliferation were analyzed by real-time RT-PCR and MTT assays, respectively. Ovarian KLF4 expression and Bcl-2/Bax ratios were downregulated in most cases of advanced EOC. In addition, KLF4 overexpression in ovarian cancer cells increased the Bcl-2/Bax ratio. However, MTT analysis indicated that the overexpression of KLF4 had no effect on the proliferation of ovarian cancer cells. The inactivation of KLF4 is frequently observed in ovarian cancers and a reduced expression of KLF4 in the ovarian cancers may lead to a reduction in the Bcl-2/Bax ratio. The latter has a role in predicting cancer grade, although its exact role in ovarian carcinogenesis requires clarification.