Apical constriction in distal visceral endoderm cells initiates global, collective cell rearrangement in embryonic visceral endoderm to form anterior visceral endoderm.
Apical constriction in distal visceral endoderm cells initiates global, collective cell rearrangement in embryonic visceral endoderm to form anterior visceral endoderm.
复制标题
远端内脏内胚层细胞的顶端收缩引发胚胎内脏内胚层的整体集体细胞重排，形成前内脏内胚层。
DOI：
10.1016/j.ydbio.2017.07.004
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Shioi;G.;Hoshino;H.;Abe;T.;Kiyonari;H.;Nakao;K.;Meng;W.;Furuta;Y.;Fujimori;T.;and Aizawa;S.
采用小鼠细胞系r26 - h2b - egfpandr26 - pha7 - egfp7分别观察由远端内脏内胚层（DVE）形成的前内脏内胚层（AVE）细胞的行为，以观察细胞核和粘附连接。使用gr26 - h2b - egfp分析显示，单层胚胎内脏内胚层中，细胞的整体重排并不特异于DVE细胞；每一群内胚层细胞作为一个整体以旋涡运动的方式集体运动。大多数E6.5的AVE细胞不是E5.5 DVE细胞，而是位于E5.5 DVE细胞尾部的E5.5细胞，如先前报道（Hoshino et al., 2015; Takaoka et al., 2011）。在重排过程中，胚胎后内脏内胚层细胞不动，胚胎外内脏内胚层细胞不动，它们在前后轴形成过程中构成了一个独特的群体。使用gr26 - pha7 - egfp的分析表明，DVE前瞻性前部细胞的顶端表面收缩启动了胚胎内脏内胚层的整体细胞运动，从而驱动AVE的形成。
The behavior of visceral endoderm cells was examined as the anterior visceral endoderm (AVE) formed from the distal visceral endoderm (DVE) using the mouse linesR26-H2B-EGFPandR26-PHA7-EGFPto visualize cell nuclei and adherens junction, respectively. The analysis usingR26-H2B-EGFPdemonstrated global cell rearrangement that was not specific to the DVE cells in the monolayer embryonic visceral endoderm sheet; each population of the endoderm cells moved collectively in a swirling movement as a whole. Most of the AVE cells at E6.5 were not E5.5 DVE cells but were E5.5 cells that were located caudally behind them, as previously reported (Hoshino et al., 2015; Takaoka et al., 2011). In the rearrangement, the posterior embryonic visceral endoderm cells did not move, as extraembryonic visceral endoderm cells did not, and they constituted a distinct population during the process of anterior-posterior axis formation. The analysis usingR26-PHA7-EGFPsuggested that constriction of the apical surfaces of the cells in prospective anterior portion of the DVE initiated the global cellular movement of the embryonic visceral endoderm to drive AVE formation.