Neuronal transcriptome of Aplysia:: neuronal compartments and circuitry
Neuronal transcriptome of Aplysia:: neuronal compartments and circuitry
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DOI：
10.1016/j.cell.2006.09.052
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发表时间：
2006-12-29
期刊：
影响因子：
64.5
通讯作者：
Kandel, Eric R.
中科院分区：
文献类型：
--
作者：
Moroz, Leonid L.;Edwards, John R.;Kandel, Eric R.
作为细胞和系统神经科学的一种成熟的模式生物，兔耳虫的分子分析由于缺乏足够的基因组信息而受到严重的阻碍。通过对来自中枢神经系统（CNS）的cDNA文库进行测序，我们已经鉴定出超过175,000个表达序列标签（est），其中19,814个是独特的神经元基因产物，占整个泛子神经元转录组的50%-70%。我们在三个层面上描述了转录组的特征：(1)中枢神经系统；(2)通过分析感觉、运动和血清素能调节神经元，分析一种简单行为的基本组成部分：鳃退缩反射；(3)单个神经元的过程。除了增加了两个数量级的可用基因序列的数量外，这个收集代表了任何一种浮游动物成员可用的最大数据库，因此为这种高度成功的多样化谱系所使用的进化策略提供了额外的见解，这是三个被提出的双侧动物超级群之一。
Molecular analyses of Aplysia, a well-established model organism for cellular and systems neural science, have been seriously handicapped by a lack of adequate genomic information. By sequencing cDNA libraries from the central nervous system (CNS), we have identified over 175,000 expressed sequence tags (ESTs), of which 19,814 are unique neuronal gene products and represent 50%-70% of the total Aplysia neuronal transcriptome. We have characterized the transcriptome at three levels: (1) the central nervous system, (2) the elementary components of a simple behavior: the gill-withdrawal reflex-by analyzing sensory, motor, and serotonergic modulatory neurons, and (3) processes of individual neurons. In addition to increasing the amount of available gene sequences of Aplysia by two orders of magnitude, this collection represents the largest database available for any member of the Lophotrochozoa and therefore provides additional insights into evolutionary strategies used by this highly successful diversified lineage, one of the three proposed super-clacles of bilateral animals.