Ultrastructural localization of tyrosine hydroxylase in noradrenergic neurons of brain.
Ultrastructural localization of tyrosine hydroxylase in noradrenergic neurons of brain.
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DOI：
10.1073/pnas.72.2.659
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发表时间：
1975-02
影响因子：
11.1
通讯作者：
V. Pickel;T. Joh;D. Reis
中科院分区：
文献类型：
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作者：
V. Pickel;T. Joh;D. Reis
酪氨酸羟化酶（EC 1.14.16.2）是催化儿茶酚胺生物合成限速步骤的酶，用过氧化物酶-抗过氧化物酶免疫组织化学方法标记的酪氨酸羟化酶特异性抗体，在电镜下定位于大鼠脑蓝斑核去甲肾上腺素能神经元内。标记的细胞体及其过程很容易与未染色的神经细胞成分区分开来。羟化酶仅在神经元细胞质中可见。其在突起中的分布与在胞体中的分布不同。在轴突和树突的纵剖面上，过氧化物反应产物呈纤维状，与质膜平行排列。在横截面上，标记结构的直径为220 a，并且在过程中呈有序分布。过氧化物酶染色结构的大小和分布表明它们是神经小管。核周细胞质弥漫性染色；内质网膜和高尔基体膜染色最强烈，溶酶体和线粒体不染色。酪氨酸羟化酶的超微结构定位与生化数据一致，表明该酶在细胞体和过程中以不同的状态存在。中枢神经元神经递质合成酶及其过程的超微结构鉴定可能为绘制脑内可识别神经元上化学特异性突触的分布提供有用的工具。
Tyrosine hydroxylase (EC 1.14.16.2), the enzyme catalyzing the rate-limiting step in catecholamine biosynthesis, was localized by electron microscopy within noradrenergic neurons of the nucleus locus coeruleus of the rat brain with a specific antibody to tyrosine hydroxylase labeled by the peroxidase-antiperoxidase immunohistochemical method. Labeled cell bodies and their processes were easily distinguished from unstained neuronal elements in the neuropil. The hydroxylase was only seen in the neuronal cytoplasm. Its distribution in processes was different from that in cell soma. In longitudinal sections of axons and dendrites, the peroxides reaction product appeared as fiber-like strands aligned parallel with the plasma membrane. In cross section, the labeled structures had a diameter of 220 A and exhibited an orderly distribution within the processes. The size and distribution of the peroxidase-stained structures suggest that they are neurotubules. In the perikarya, the cytoplasm was diffusely stained; the reaction was most intense on membranes of endoplasmic reticulum and Golgi apparatus, whereas lysosomes and mitochondria did not stain. The ultrastructural localization of tyrosine hydroxylase is consistent with biochemical data suggesting that the enzyme exists in different states in cell body and processes. The ultrastructural identification of enzymes subserving synthesis of neurotransmitters in central neurons and their processes may provide a useful tool in mapping the distribution of chemically specific synapses on identifiable neurons in brain.