AUTOLOGOUS PLASMIN FOR PHARMACOLOGIC VITREOLYSIS PREPARED 1 HOUR BEFORE SURGERY
AUTOLOGOUS PLASMIN FOR PHARMACOLOGIC VITREOLYSIS PREPARED 1 HOUR BEFORE SURGERY
复制标题
手术前 1 小时制备用于药物玻璃体溶解的自体纤溶酶
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
M. Vispi
中科院分区：
文献类型：
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作者：
S. Rizzo;G. Pellegrini;F. Benocci;C. Belting;U. Baicchi;M. Vispi
目的：目的评价玻璃体腔内注射自体纤溶酶（APE）诱导玻璃体后脱离（PVD）的安全性和有效性。术前1h用改良法测APE。方法：术前1h取自体全血，离心后与链激酶孵育，获得APE。手术前20分钟在玻璃体腔内注射APE。该手术应用于20例因玻璃体视网膜牵引引起的黄斑皱褶、黄斑裂孔和黄斑水肿而接受玻璃体切除术的患者。术中对PVD状态进行分级。比较了通过该方法获得的纤溶酶浓度和来自每个患者的血浆的纤溶酶原滴度。在术前和术后1、6、12和24小时控制活化部分凝血活酶时间，以评价由于纤溶酶的全身吸收引起的凝血变化。结果：APE的准备过程简单，平均45 min.术中观察到17只眼（85%）的PVD伴明显的韦斯环。平均纤溶酶活性为0.26 IU/0.2 mL活化血浆。部分凝血活酶时间与术前值相比没有任何改变。结论：该方法获得的APE可导致PVD，有利于玻璃体皮质的完全切除。玻璃体内注射不会导致全身凝血功能的改变。
Purpose: To evaluate the safety and efficacy of intravitreal injection of autologous plasmin enzyme (APE) in inducing a posterior vitreous detachment (PVD). APE was obtained by a modified method 1 hour before surgery. Methods: APE was obtained by centrifugation of autologous whole blood from the patients 1 hour before surgery and by incubation with streptokinase. APE was injected in the vitreous cavity 20 minutes before surgery. This procedure was applied for 20 patients who underwent vitrectomy for macular pucker, macular hole, and macular edema due to vitreoretinal traction. The status of PVD was graded intraoperatively. The plasmin concentration obtained by this method and the plasminogen titer of the plasma from each patient were compared. Activated partial thromboplastin time was controlled before surgery and 1, 6, 12, and 24 hours after surgery to evaluate alteration of the coagulation due to systemic absorption of the plasmin. Results: Preparation of APE was easy and took on average 45 minutes for all patients. A PVD with an evident Weiss ring was observed during surgery in 17 eyes (85%). The average plasmin activity was 0.26 IU/0.2 mL activated plasma. The partial thromboplastin time did not show any alteration in any of the patients with respect to preoperative values. Conclusion: APE obtained by this method can lead to a PVD and facilitate the complete remotion of vitreous cortex. The intravitreal injection does not lead to alteration of systemic blood coagulation.
DOI：
10.1001/archopht.1993.01090060139038
发表时间：
1993
期刊：
Archives of ophthalmology (Chicago, Ill. : 1960)
影响因子：
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作者：
Verstraeten,TC;Chapman,C;Hartzer,M;Winkler,BS;Trese,MT;Williams,GA
通讯作者：
Williams,GA