Expression and distribution of PPP2R5C gene in leukemia.
Expression and distribution of PPP2R5C gene in leukemia.
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PPP2R5C基因在白血病中的表达及分布
DOI：
10.1186/1756-8722-4-21
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发表时间：
2011-05-06
影响因子：
28.5
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Zheng H;Chen Y;Chen S;Niu Y;Yang L;Li B;Lu Y;Geng S;Du X;Li Y
最近，我们在分子水平的新型染色体易位t（14; Q11; Q32）中阐明了Sézary综合征，这导致了从TRAJ7重排成PPP2R5C基因的重排。磷酸酶2a（PP2A）在细胞增殖，分化和转化中起着至关重要的作用。来自77例新白血病患者，26例完全缓解的白血病患者（CR）和20个健康个体通过实时PCR，并通过RT-PCR鉴定出PPP2R5C的不同变体。
与健康对照相比，在AML，CML，T-ALL和B-CLL组中发现了PPP2R5C的明显更高的表达。与从头CML组相比，CML-CR组中PPP2R5C的表达水平显着改善可以在健康样本以及所有白血病样本中检测到PPP2R5C的变体。
PPP2R5C在T细胞恶性肿瘤以及髓样白血病细胞中的过表达可能与其增殖和分化有关。
Recently, we clarified at the molecular level novel chromosomal translocation t(14;14)(q11;q32) in a case of Sézary syndrome, which caused a rearrangement from TRAJ7 to the PPP2R5C gene. PPP2R5C is one of the regulatory B subunits of protein phosphatase 2A (PP2A). It plays a crucial role in cell proliferation, differentiation, and transformation. To characterize the expression and distribution of five different transcript variants of the PPP2R5C gene in leukemia, we analyzed the expression level of PPP2R5C in peripheral blood mononuclear cells from 77 patients with de novo leukemia, 26 patients with leukemia in complete remission (CR), and 20 healthy individuals by real-time PCR and identified the different variants of PPP2R5C by RT-PCR. Significantly higher expression of PPP2R5C was found in AML, CML, T-ALL, and B-CLL groups in comparison with healthy controls. High expression of PPP2R5C was detected in the B-ALL group; however, no significant difference was found compared with the healthy group. The expression level of PPP2R5C in the CML-CR group decreased significantly compared with that in the de novo CML group and was not significantly different from the level in the healthy group. By using different primer pairs that covered different exons, five transcript variants of PPP2R5C could be identified. All variants could be detected in healthy samples as well as in all the leukemia samples, and similar frequencies and distributions of PPP2R5C were indicated. Overexpression of PPP2R5C in T-cell malignancy as well as in myeloid leukemia cells might relate to its proliferation and differentiation. Investigation of the effect of target inhibition of this gene might be beneficial to further characterization of molecular mechanisms and targeted therapy in leukemia.
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影响因子：
28.5
作者：
Braoudaki M;Papathanassiou C;Katsibardi K;Tourkadoni N;Karamolegou K;Tzortzatou-Stathopoulou F
通讯作者：
Tzortzatou-Stathopoulou F
影响因子：
28.5
作者：
Wang L;Zhu K;Zha X;Chen S;Yang L;Chen S;Li Y
通讯作者：
Li Y
影响因子：
4.4
作者：
Muneer, S;Ramalingam, V;Kamibayashi, C
通讯作者：
Kamibayashi, C
影响因子：
8
作者：
Shouse, G. P.;Nobumori, Y.;Liu, X.
通讯作者：
Liu, X.
影响因子：
5.3
作者：
Shouse, Geoffrey P.;Cai, Xin;Liu, Xuan
通讯作者：
Liu, Xuan