Hierarchical 2D to 3D micro/nano-histology of human dental caries lesions using light, X-ray and electron microscopy
Hierarchical 2D to 3D micro/nano-histology of human dental caries lesions using light, X-ray and electron microscopy
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DOI：
10.1016/j.matdes.2022.110829
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发表时间：
2022-08-01
影响因子：
8.4
通讯作者：
Korsunsky, Alexander M.
中科院分区：
文献类型：
--
作者：
Besnard, Cyril;Marie, Ali;Korsunsky, Alexander M.
龋齿是一种广泛存在的疾病，它是通过不均匀溶解破坏牙齿表层的牙釉质而引起的。传统组织学通过其光学外观识别龋齿病变内的不同区域，但无法量化潜在的纳米级结构变化作为特定位置的功能，从而阻碍了对脱矿过程的更好理解。我们使用不同的成像方式、分辨率和视场进行详细的搭配分析。聚焦离子束扫描电子显微镜（FIB-SEM）可以揭示磨铣微体积内的地下3D纳米结构，而x射线断层扫描可以在大体积上进行破坏性最小的3D成像。这些技术的相关组合揭示了作为位置函数的搪瓷棒、棒间物质、鞘、晶体和空隙的精细细节。病变体内、前部附近和表面的牙釉质脱矿程度在3D中可视化和量化。因此，我们建立了牙齿三维纳米组织学的范例，作为定量评估龋齿引起的结构改变的先进平台。CO 2022作者。Elsevier Ltd.出版。这是一篇基于CC BY许可（http:// creativecommons.org/licenses/by/4.0/）的开放获取文章。
Dental caries is a widespread disease that proceeds by damaging superficial tooth enamel by heterogeneous dissolution. Conventional histology identifies different zones within carious lesions by their optical appearance, but fails to quantify the underlying nanoscale structural changes as a function of specific location, impeding better understanding of the demineralisation process. We employ detailed collocative analysis using different imaging modalities, resolutions and fields of view. Focused ion beam-scanning electron microscopy (FIB-SEM) reveals subsurface 3D nanostructure within milled micro-sized volumes, whilst X-ray tomography allows minimally destructive 3D imaging over large volumes. Correlative combination of these techniques reveals fine detail of enamel rods, inter-rod substance, sheaths, crystallites and voids as a function of location. The degree of enamel demineralisation within the body of the lesion, near its front, and at the surface is visualized and quantified in 3D. We thus establish the paradigm of dental 3D nano-histology as an advanced platform for quantitative evaluation of caries-induced structural modification.CO 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).