Hyperoxia inhibits proliferation of cultured rat tracheal smooth muscle cells.
Hyperoxia inhibits proliferation of cultured rat tracheal smooth muscle cells.
复制标题
高氧抑制培养的大鼠气管平滑肌细胞的增殖。
DOI：
10.1152/ajplung.1994.267.2.l101
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Evans,JN
中科院分区：
文献类型：
--
作者：
Absher,M;Makrides,W;Shapiro,P;Evans,JN
肺暴露于高氧导致结构和细胞损伤，随后是广泛的组织重塑。利用暴露于高氧条件或外源添加的氧化剂的分离细胞的体外模型可能是有害的或刺激性的，这取决于特定的细胞类型和暴露水平和持续时间。在本研究中，培养的大鼠气管平滑肌细胞的增殖抑制氧浓度为40和70%相比，“常氧”浓度为21%。暴露于70%的氧气对细胞有肥大作用，如细胞蛋白质含量增加所示，而暴露于21%的氧气的细胞没有显示蛋白质含量增加。外源性氧化剂H2O2在浓度> 3 μ M时可完全抑制气管平滑肌细胞的生长。需要更高浓度的H2O2才能抑制血管平滑肌细胞和大鼠肺成纤维细胞的增殖。气道平滑肌细胞对氧和氧化剂的敏感性增加可能是高氧肺损伤早期事件的重要因素。
Exposure of the lung to elevated oxygen leads to structural and cellular injury followed by extensive tissue remodeling. In vitro models utilizing isolated cells exposed to hyperoxic conditions or exogenously added oxidants may be injurious or stimulatory depending on the specific cell type and level and duration of exposure. In the present study, proliferation of cultured rat tracheal smooth muscle cells was inhibited by oxygen concentrations of 40 and 70% compared with a "normoxic" concentration of 21%. Exposure to 70% oxygen had a hypertrophic effect on the cells, as indicated by increased cellular protein content, whereas cells exposed to 21% oxygen did not show increased protein content. Exogenously added oxidant, H2O2, resulted in complete inhibition of growth of tracheal smooth muscle cells at concentrations > 3 microM. Much higher concentrations of H2O2 were required to inhibit proliferation of vascular smooth muscle cells and rat lung fibroblasts. The heightened sensitivity of airway smooth muscle cells to oxygen and oxidants may be an important factor in the early events following hyperoxia-induced lung injury.