A delivery system for field application of paratransgenic control
A delivery system for field application of paratransgenic control
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DOI：
10.1186/s12896-015-0175-3
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发表时间：
2015-06-23
影响因子：
3.5
通讯作者：
Durvasula, Ravi
中科院分区：
文献类型：
--
作者：
Arora, Arinder K.;Forshaw, Adam;Durvasula, Ravi
背景资料：作为化学农药的替代品，副转基因依赖于节肢动物载体的共生细菌的转化，以递送破坏病原体传播的分子。十多年来，由于对将转化的微生物引入环境中的监管问题，副转基因一直是基于实验室的奋进。为了方便现场应用的paratransgenic战略，风险缓解的方法，解决环境污染和基因spreads.Results：使用生物聚合物的操作，我们引入了一种新的微胶囊化平台的遏制和有针对性的交付工程菌的肠道疾病传播节肢动物。我们证明了EPA批准的Pantoea agglomerans E325在副转基因系统中靶向递送的原理的第一个证据，以控制皮尔斯病在模拟田间条件下由玻璃翅神枪手（Homalodisca vitripennis）传播。工程微胶囊可以解决有关重组细菌的遏制和外源遗传物质的环境传播的监管问题，并可能代表将副转基因科学从实验室转化到实地的重要一步。我们将首次介绍，一种微囊化策略，将重组细菌递送至昆虫，并证明细菌靶向释放至昆虫的生理相关区域，昆虫内脏这是解决与重组细菌的现场应用相关的问题的第一步。工程化微粒可以通过充当重组细菌与环境之间的屏障来减少环境污染、水平基因转移和与天然物种的竞争。
Background: As an alternative to chemical pesticides, paratransgenesis relies on transformation of symbiotic bacteria of an arthropod vector to deliver molecules that disrupt pathogen transmission. For over a decade paratransgenesis has remained a laboratory-based endeavor owing to regulatory concerns regarding introduction of transformed microorganisms into the environment. To facilitate field application of paratransgenic strategies, risk mitigation approaches that address environmental contamination and gene spread must be developed.Results: Using biopolymer manipulation, we introduce a novel microencapsulation platform for containment and targeted delivery of engineered bacteria to the gut of a disease-transmitting arthropod. We demonstrate the first proof of principle of targeted delivery of EPA-approved Pantoea agglomerans E325 in a paratransgenic system to control spread of Pierce's Disease by glassy-winged sharpshooters, (Homalodisca vitripennis) under simulated field conditions. Engineered microcapsules may address regulatory concerns regarding containment of recombinant bacteria and environmental spread of foreign genetic material and may represent an important step in translating paratransgenic science beyond the lab and into the field.Conclusions: We present, for the first time, a microencapsulation strategy to deliver recombinant bacteria to an insect and demonstrate targeted release of bacteria into the physiologically relevant region of the insect gut. This is a first step toward addressing concerns related to field application of recombinant bacteria. Engineered microparticles may decrease environmental contamination, horizontal gene transfer and competition with native species by acting as a barrier between recombinant bacteria and the environment.