TARGETED DISRUPTION OF METALLOTHIONEIN-I AND METALLOTHIONEIN-II GENES INCREASES SENSITIVITY TO CADMIUM
TARGETED DISRUPTION OF METALLOTHIONEIN-I AND METALLOTHIONEIN-II GENES INCREASES SENSITIVITY TO CADMIUM
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DOI：
10.1073/pnas.91.2.584
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发表时间：
1994-01-18
影响因子：
11.1
通讯作者：
PALMITER, RD
中科院分区：
文献类型：
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作者：
MASTERS, BA;KELLY, EJ;PALMITER, RD
我们灭活了胚胎干细胞中的小鼠金属硫蛋白（MT）-I和MT-II基因，并产生了这些突变等位基因的纯合子小鼠。这些小鼠在正常实验室条件下饲养时存活并正常繁殖。然而，他们更容易受到镉的肝中毒。这证明这些广泛表达的MT对发育不是必需的，但它们确实可以防止镉毒性。这些小鼠提供了用于测试MT在体内的其他拟议功能的手段。
We inactivated the mouse metallothionein (MT)-I and MT-II genes in embryonic stem cells and generated mice homozygous for these mutant alleles. These mice were viable and reproduced normally when reared under normal laboratory conditions. They were, however, more susceptible to hepatic poisoning by cadmium. This proves that these widely expressed MTs are not essential for development but that they do protect against cadmium toxicity. These mice provide a means for testing other proposed functions of MT in vivo.