Trans-acting regulatory variation in Saccharomyces cerevisiae and the role of transcription factors
Trans-acting regulatory variation in Saccharomyces cerevisiae and the role of transcription factors
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DOI：
10.1038/ng1222
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发表时间：
2003-09-01
期刊：
影响因子：
30.8
通讯作者：
Kruglyak, L
中科院分区：
文献类型：
--
作者：
Yvert, G;Brem, RB;Kruglyak, L
自然遗传变异可导致基因表达的显著差异，但对影响基因调控的多态性知之甚少。我们分析了酿酒酵母的实验室和野生菌株之间的交叉监管的变化。聚类和连锁分析定义了共调节基因组和参与其调节的位点。大多数表达差异映射到反式作用位点。定位克隆和功能分析表明，在GPA 1和AMN 1的多态性影响参与信息素反应和子细胞分离的基因的表达，分别。我们还询问了特定类别的基因是否更有可能包含反式调节多态性。值得注意的是，转录因子没有显示富集，并且反式调节变异似乎广泛地分散在具有不同分子功能的基因类别中。
Natural genetic variation can cause significant differences in gene expression, but little is known about the polymorphisms that affect gene regulation. We analyzed regulatory variation in a cross between laboratory and wild strains of Saccharomyces cerevisiae. Clustering and linkage analysis defined groups of coregulated genes and the loci involved in their regulation. Most expression differences mapped to trans-acting loci. Positional cloning and functional assays showed that polymorphisms in GPA1 and AMN1 affect expression of genes involved in pheromone response and daughter cell separation, respectively. We also asked whether particular classes of genes were more likely to contain trans-regulatory polymorphisms. Notably, transcription factors showed no enrichment, and trans-regulatory variation seems to be broadly dispersed across classes of genes with different molecular functions.