Quantum-dot nanocrystals for ultrasensitive biological labeling and multicolor optical encoding
Quantum-dot nanocrystals for ultrasensitive biological labeling and multicolor optical encoding
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DOI：
10.1117/1.1506706
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发表时间：
2002-10-01
影响因子：
3.5
通讯作者：
Nie, SM
中科院分区：
文献类型：
--
作者：
Gao, XH;Chan, WCW;Nie, SM
尺寸为2-6 nm的半导体纳米颗粒不仅因为其尺寸可调的特性，而且因为它们与生物大分子(如核酸和蛋白质)的尺寸相似，所以引起了人们的极大兴趣。这种相似性可以使纳米材料与生物分子相结合，这将在医疗诊断、靶向治疗和高通量药物筛选中得到应用。在这里，我们报道了在制备高发光和生物相容的CdSe量子点(QD)以及合成尺寸在100 nm-10微米范围内的QD编码微球和纳米球方面的新进展。我们发现，硫化锌包裹的量子点的光学性质对环境因素如pH和二价阳离子非常敏感，这使得量子点在分子传感方面具有潜在的应用前景。我们还表明，化学修饰的蛋白质可以用来包覆水溶性量子点的表面，恢复它们的荧光，并为生物偶联提供官能团。对于多路光学编码，我们已经制备了尺寸与哺乳动物细胞相似的大微珠和大小与病毒相似的小纳米棒。(C)2002年光学仪器工程师学会。
Semiconductor nanoparticles in the size range of 2-6 nm are of great current interest, not only because of their size-tunable properties but also because of their dimensional similarity with biological macromolecules (e.g., nucleic acids and proteins). This similarity could allow an integration of nanomaterials with biological molecules, which would have applications in medical diagnostics, targeted therapeutics, and high-throughput drug screening. Here we report new developments in preparing highly luminescent and biocompatible CdSe quantum dots (QDs), and in synthesizing QD-encoded micro- and nano-beads in the size range of 100 nm-10 mum. We show that the optical properties of ZnS-capped CdSe quantum dots are sensitive to environmental factors such as pH and divalent cations, leading to the potential use of quantum dots in molecular sensing. We also show that chemically modified proteins can be used to coat the surface of water-soluble QDs, to restore their fluorescence, and to provide functional groups for bioconjugation. For multiplexed optical encoding, we have prepared large microbeads with sizes similar to that of mammalian cells, and small nanobeads with sizes similar to that of viruses. (C) 2002 Society of Photo-Optical Instrumentation Engineers.