Simultaneous determination of plant hormones in peach based on dispersive liquid-liquid microextraction coupled with liquid chromatography-ion trap mass spectrometry
Simultaneous determination of plant hormones in peach based on dispersive liquid-liquid microextraction coupled with liquid chromatography-ion trap mass spectrometry
复制标题
分散液液微萃取-液相色谱-离子阱质谱联用测定桃中植物激素
DOI：
10.1016/j.jchromb.2015.04.014
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发表时间：
2015-06-15
影响因子：
3
通讯作者：
Li, Jianrong
中科院分区：
文献类型：
--
作者：
Lu, Qiaomei;Zhang, Wenmin;Li, Jianrong
Fruit development is influenced greatly by endogenous hormones including salicylic acid (SA) and abscisic acid (ABA). Mass spectrometry with high sensitivity has become a routine technology to analyze hormones. However, pretreatment of plant samples remains a difficult problem. Thus, dispersive liquid-liquid microextraction (DLLME) was used to concentrate trace plant hormones before liquid chromatography-ion trap mass spectrometry (LC-ITMS) analysis. Standard curves were linear within the ranges of 0.5-50, 0.2-20 ng/mL for SA and ABA, respectively. The correlation coefficients were greater than 0.9995 with recoveries above 87.5%. The limits of detection were 0.2 ng/mL for SA and 0.1 ng/mL for ABA in spiked water solution, respectively (injection 20 mu L). The successful analysis of SA and ABA in fruit samples indicated our DLLME-LC-ITMS approach was efficient, allowing reliable quantification of both two compounds from very small amounts of plant material. Moreover, this research revealed the relationship between SA and ABA content and development of peach fruit at different growth stages. (C) 2015 Elsevier B.V. All rights reserved.
