Improved herbivore resistance in cultivated tomato with the sesquiterpene biosynthetic pathway from a wild relative
Improved herbivore resistance in cultivated tomato with the sesquiterpene biosynthetic pathway from a wild relative
复制标题
DOI：
10.1073/pnas.1208756109
复制
发表时间：
2012-12-04
影响因子：
11.1
通讯作者：
Schuurink, Robert C.
中科院分区：
文献类型：
--
作者：
Bleeker, Petra M.;Mirabella, Rossana;Schuurink, Robert C.
番茄育种在提高果实质量和数量方面非常有效，但并没有专注于提高对植食动物的抗性。将野生番茄中7-表姜烯的生物合成途径导入温室栽培品种，以获得抗植食性。7-表姜烯是一种具有毒性和驱避特性的特定倍半萜，其产生并储存在腺毛中。我们鉴定了7-表姜烯合酶（ShZIS），它属于一类新的倍半萜烯脱氢酶，在质体中专门使用Z-Z-法尼基-二磷酸（zFPP），可能是通过共同祖先的新功能化产生的。ShZIS和zFPP酶在栽培番茄的腺毛中的表达导致7-表姜烯的产生。这些番茄获得了对番茄害虫的几种食草动物的抗性。因此，将这种倍半萜烯生物合成途径引入栽培番茄可以提高对食草动物的抵抗力。
Tomato breeding has been tremendously efficient in increasing fruit quality and quantity but did not focus on improving herbivore resistance. The biosynthetic pathway for the production of 7-epizingiberene in a wild tomato was introduced into a cultivated greenhouse variety with the aim to obtain herbivore resistance. 7-Epizingiberene is a specific sesquiterpene with toxic and repellent properties that is produced and stored in glandular trichomes. We identified 7-epizingiberene synthase (ShZIS) that belongs to a new class of sesquiterpene synthases, exclusively using Z-Z-farnesyl-diphosphate (zFPP) in plastids, probably arisen through neo-functionalization of a common ancestor. Expression of the ShZIS and zFPP synthases in the glandular trichomes of cultivated tomato resulted in the production of 7-epizingiberene. These tomatoes gained resistance to several herbivores that are pests of tomato. Hence, introduction of this sesquiterpene biosynthetic pathway into cultivated tomatoes resulted in improved herbivore resistance.