Molecular characterization of flavonoid malonyltransferase from Oryza sativa
Molecular characterization of flavonoid malonyltransferase from Oryza sativa
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DOI：
10.1016/j.plaphy.2009.08.004
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发表时间：
2009-11-01
影响因子：
6.5
通讯作者：
Ahn, Joong-Hoon
中科院分区：
文献类型：
--
作者：
Kim, Dea Hwan;Kim, Seong Kyong;Ahn, Joong-Hoon
本研究从水稻中克隆了一个类黄酮丙二酸基转移酶(OsMaT-2)，并通过亲和层析纯化了重组蛋白OsMaT-2。OsMaT-2利用多种黄酮苷，包括黄酮苷、黄酮苷、黄酮苷和异黄酮苷作为底物，但不利用花青素。作为酰基供体，OsMaT-2仅利用丙二酰辅酶A。根据与不同的栎素3-O-糖的反应，我们确定了丙二酸化的可能位置是糖的6‘’-羟基。据我们所知，这是首次从水稻中克隆到一个黄酮型丙二酰基转移酶。(C)2009年爱思唯尔·马森公司。版权所有。
In this study, a flavonoid malonyltransferase (OsMaT-2) was cloned from Oryza sativa, and the recombinant protein OsMaT-2 was purified via affinity chromatography. OsMaT-2 utilized a variety of flavonoid glucosides, including flavanone glucosides, flavone glucosides, flavonol glucosides, and isoflavone glucosides as substrates, but did not utilize anthocyanin. As an acyl donor, OsMaT-2 utilized only malonyl-CoA. Based on reactions with various quercetin 3-O-sugars, we identified the probable position of malonylation as the 6 ''-hydroxyl group of the sugar. This is the first report, to the best of our knowledge, of the cloning of a flavonoid malonyltransferase from O. sativa. (C) 2009 Elsevier Masson SAS. All rights reserved.