Insulin binding and degradation in vascular endothelial cells: modulation by cell growth and culture organization.
Insulin binding and degradation in vascular endothelial cells: modulation by cell growth and culture organization.
复制标题
血管内皮细胞中的胰岛素结合和降解：细胞生长和培养组织的调节。
DOI：
10.1210/endo-113-1-228
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发表时间：
1983
期刊：
影响因子：
4.8
通讯作者：
E. Cerasi
中科院分区：
文献类型：
--
作者：
N. Kaiser;I. Vlodavsky;A. Tur;Z. Fuks;E. Cerasi
用单层培养的牛主动脉内皮细胞研究了胰岛素与血管内皮的相互作用及其对细胞生长和培养组织的调节。研究了三种类型的培养物：1）融合的非分裂培养物，组织化和分化为体内组织; 2）亚融合的，尚未组织化的细胞培养物，代表增殖的内皮细胞; 3）内皮细胞培养物经修饰失去其接触抑制特性，以多层生长。所有三种类型的文化表现出特异性结合的125 I-胰岛素的高和低亲和力的细胞表面受体位点，并能够降解125 I-胰岛素。将培养物预暴露于胰岛素导致细胞表面受体的可用性的时间依赖性降低（下调）。与接触抑制融合培养物相比，亚融合培养物和改良培养物中每个细胞的胰岛素结合分别高2.4倍和10倍。同样，胰岛素降解速率在近汇合和改良培养物中更高（分别为2.3倍和20倍）。亚融合培养物比融合培养物对胰岛素的下调作用更敏感。与预先暴露于50 ng/ml胰岛素后融合培养物中胰岛素结合减少40%相比，它们显示胰岛素结合减少60%。生长中的内皮细胞中胰岛素结合和降解的增加表明激素在调节内皮细胞生长中的作用，例如响应于损伤。生理浓度的激素对[3 H]胸苷掺入稀疏、血清饥饿内皮细胞的剂量依赖性刺激的观察结果进一步支持了这一点。
The interaction of insulin with the vascular endothelium and its modulation by cell growth and culture organization was studied using bovine aortic endothelial cells in monolayer cultures. Three types of cultures were investigated: 1) confluent nondividing cultures, organized and differentiated as the in vivo tissue; 2) subconfluent, not yet organized cell cultures, representing proliferating endothelium; and 3) endothelial cell cultures modified to lose their property of contact inhibition, growing in multiple layers. All three types of cultures exhibited specific binding of 125I-insulin to high and low affinity cell surface receptor sites, and were capable of degrading 125I-insulin. Preexposure of the cultures to insulin resulted in a time dependent reduction in the availability of cell surface receptors (down-regulation). Insulin binding per cell was 2.4-fold and 10-fold higher in the subconfluent and modified cultures, respectively, as compared to the contact-inhibited confluent cultures. Similarly, the rate of insulin degradation was higher in the subconfluent and modified cultures (2.3-fold and 20-fold, respectively). Subconfluent cultures were more sensitive than confluent cultures to the down-regulatory effect of insulin. They exhibited a 60% decrease in insulin binding as compared to a 40% decrease in confluent cultures after preexposure to 50 ng/ml insulin. The increase in insulin binding and degradation in growing endothelial cells suggests a role for the hormone in the regulation of endothelial cell growth, e.g. in response to injury. This was further supported by the observation of a dose-dependent stimulation of [3H]thymidine incorporation into sparse, serum-starved endothelial cells by physiological concentrations of the hormone.
增殖刺激活性与牛内皮细胞的相互作用：肺循环和体循环原代、传代和克隆培养物的比较研究。
影响因子：
4.8
作者：
Bar,RS;Goldsmith,JC;Rechler,MM;Peacock,ML;Nissley,SP
通讯作者：
Nissley,SP