Expression of soybean lectin in transgenic tobacco results in enhanced resistance to pathogens and pests
Expression of soybean lectin in transgenic tobacco results in enhanced resistance to pathogens and pests
复制标题
转基因烟草中大豆凝集素的表达可增强对病原体和害虫的抵抗力
DOI：
10.1016/j.plantsci.2013.06.001
复制
发表时间：
2013-10-01
期刊：
影响因子：
5.2
通讯作者：
Gao, Xuewen
中科院分区：
文献类型：
--
作者：
Guo, Peipei;Wang, Yu;Gao, Xuewen
Lectins are proteins of non-immune origin that specifically interact with carbohydrates, known to play important roles in the defense system of plants. In this study, in order to study the function of a new soybean lectin (SBL), the corresponding encoding gene lec-s was introduced into tobacco plants via Agrobacterium-mediated transformation. Southern blot analyses had revealed that the lec-s gene was stable integrated into the chromosome of the tobacco. The results of the reverse transcription polymerase chain reaction (RT-PCR) also indicated that the lec-s gene in the transgenic tobacco plants could be expressed under the control of the constitutive CaMV35S promoter. Evaluation agronomic of the performance had showed that the transgenic plants could resist to the infection of Phytophthora nicotianae. Insect bioassays using detached leaves from transgenic tobacco plants demonstrated that the ectopically expressed SBL significantly (P.0.05) reduced the weight gain of larvae of the beet armyworm (Spodoptera exigua). Further on, the lectins retarded the development of the larvae and their metamorphosis. These findings suggest that soybean lectins have potential as a protective agent against pathogens and insect pests through a transgenic approach. (c) 2013 Elsevier Ireland Ltd. All rights reserved.
