STEREOLOGICAL CORRECTION PROCEDURES FOR ESTIMATING TRUE VOLUME PROPORTIONS FROM BIASED SAMPLES
STEREOLOGICAL CORRECTION PROCEDURES FOR ESTIMATING TRUE VOLUME PROPORTIONS FROM BIASED SAMPLES
复制标题
DOI：
10.1111/j.1365-2818.1973.tb04628.x
复制
发表时间：
1973-01-01
期刊：
影响因子：
--
通讯作者：
CRUZ, LM
中科院分区：
文献类型：
--
作者：
MAYHEW, TM;CRUZ, LM
组分体积比例的实验评价可能需要引入组分偏倚采样方法，以促进细胞类型的明确识别。巨噬细胞、神经元和淋巴细胞-浆细胞家族的细胞肯定是这种情况。使用这些系统，数学校正是必要的，以便可以从有偏数据计算出可靠的形态测量信息。所采用的校正程序的类型将在某种程度上取决于感兴趣的组分（细胞核、核仁）与其包含体积（细胞、细胞核）之间的空间关系。本文导出了空间同心度和偏心度特殊情况下的修正公式。通过应用于一些著名的模型系统来说明它们的有用性，并提供了一个参考表，用于从观察到的（有偏差的）数据中获得适当的真实体积比例。从而提高了分量偏置形态测量方法的可靠性。
Experimental evaluation of component volume proportions may require the introduction of a component‐biased sampling approach in order to facilitate the unequivocal identification of cell type. Such is certainly the case for macrophages, neurons and cells of the lymphocyte‐plasma cell family. With these systems, mathematical corrections are necessary so that reliable morphometric information can be computed from the biased data. The type of correction procedure adopted will depend, to some extent, on the spatial relationships between the component of interest (nucleus, nucleolus) and its containing volume (cell, nucleus). In this communication, correction formulae for the special cases of spatial concentricity and eccentricity are derived. Their usefulness is illustrated by application to a number of well‐known model systems, and a reference table for obtaining the appropriate true volume proportion from observed (biased) data is provided. The reliability of the component‐biased morphometric approach is thereby increased.