Indocyanine Green Loaded Reduced Graphene Oxide for In Vivo Photoacoustic/Fluorescence Dual-Modality Tumor Imaging.
Indocyanine Green Loaded Reduced Graphene Oxide for In Vivo Photoacoustic/Fluorescence Dual-Modality Tumor Imaging.
复制标题
DOI：
10.1186/s11671-016-1288-x
复制
发表时间：
2016-12
影响因子：
--
通讯作者：
Song L
中科院分区：
文献类型：
--
作者：
Chen J;Liu C;Zeng G;You Y;Wang H;Gong X;Zheng R;Kim J;Kim C;Song L
基于多功能纳米复合材料的多模态成像具有很大的希望，从根本上提高生物医学成像的能力。具体而言，光声和荧光双模态成像由于其非侵入性和两种模态在成像分辨率、深度、灵敏度和速度方面的互补性而获得了很大的兴趣。本文采用一种绿色、简便的方法，合成了吲哚菁绿色（ICG）负载的聚乙二醇（PEG）化还原纳米氧化石墨烯纳米复合材料（rNGO-PEG/ICG），作为一种新型的荧光和光声双模态成像对比剂。该纳米复合材料在体外和体内均显示出最小的毒性和优异的光声/荧光信号。与游离ICG相比，纳米复合物具有更好的稳定性、更长的血液循环时间和更好的上级被动肿瘤靶向能力。体内研究表明，静脉注射rNGO-PEG/ICG在小鼠体内的循环时间可持续6 h，而1天后，除肿瘤区域外，在任何主要器官中均未发现rNGO-PEG/ICG明显蓄积。所展示的高荧光/光声双重对比度，连同其低毒性和优异的循环寿命，表明合成的rNGO-PEG/ICG可以是癌症的早期诊断和图像引导治疗/手术的进一步转化研究的有希望的候选者。本文的在线版本（doi：10.1186/s11671-016-1288-x）包含补充材料，可供授权用户使用。
Multimodality imaging based on multifunctional nanocomposites holds great promise to fundamentally augment the capability of biomedical imaging. Specifically, photoacoustic and fluorescence dual-modality imaging is gaining much interest because of their non-invasiveness and the complementary nature of the two modalities in terms of imaging resolution, depth, sensitivity, and speed. Herein, using a green and facile method, we synthesize indocyanine green (ICG) loaded, polyethylene glycol (PEG)ylated, reduced nano-graphene oxide nanocomposite (rNGO-PEG/ICG) as a new type of fluorescence and photoacoustic dual-modality imaging contrast. The nanocomposite is shown to have minimal toxicity and excellent photoacoustic/fluorescence signals both in vitro and in vivo. Compared with free ICG, the nanocomposite is demonstrated to possess greater stability, longer blood circulation time, and superior passive tumor targeting capability. In vivo study shows that the circulation time of rNGO-PEG/ICG in the mouse body can sustain up to 6 h upon intravenous injection; while after 1 day, no obvious accumulation of rNGO-PEG/ICG is found in any major organs except the tumor regions. The demonstrated high fluorescence/photoacoustic dual contrasts, together with its low toxicity and excellent circulation life time, suggest that the synthesized rNGO-PEG/ICG can be a promising candidate for further translational studies on both the early diagnosis and image-guided therapy/surgery of cancer. The online version of this article (doi:10.1186/s11671-016-1288-x) contains supplementary material, which is available to authorized users.