Fission yeast Tup1-like repressors repress chromatin remodeling at the fbp1+ promoter and the ade6-M26 recombination hotspot.
Fission yeast Tup1-like repressors repress chromatin remodeling at the fbp1+ promoter and the ade6-M26 recombination hotspot.
复制标题
裂殖酵母 Tup1 样阻遏物抑制 fbp1 启动子和 ade6-M26 重组热点处的染色质重塑。
DOI：
10.1093/genetics/165.2.505
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发表时间：
2003
期刊：
影响因子：
3.3
通讯作者：
Ohta,Kunihiro
中科院分区：
文献类型：
--
作者：
Hirota,Kouji;Hoffman,CharlesS;Shibata,Takehiko;Ohta,Kunihiro
染色质重塑在基因表达和重组的调控中起着至关重要的作用。裂殖酵母fbp 1+基因的转录和减数分裂重组热点6-M26（M26）的重组均受cAMP反应元件（CRE）样序列和CREB/ATF型转录因子Atf 1·Pcr 1的调控。Tup 11和Tup 12蛋白是Saccharomyces gallae Tup 1辅阻遏物的裂殖酵母对应物，参与葡萄糖对fbp 1+转录的阻遏。我们分析了Tup 1样辅阻遏物在fbp 1+启动子和M26热点周围的染色质调控中的作用。我们发现，fbp 1+的两个调控元件周围的染色质结构在去阻遏条件下与bbp 1+转录的强烈激活相一致。在阻遏条件下生长的具有tup 11 Δ tup 12 Δ双缺失的菌株表现出与在去阻遏条件下生长的野生型细胞相关的染色质状态。有趣的是，rst 2+（编码由cAMP依赖性激酶控制的转录因子）的缺失减轻了染色质调节中的thetup 11 Δ tup 12 Δ缺陷，但没有减轻转录抑制中的缺陷。atup 11 Δ tup 12 Δ突变体的有丝分裂培养物中M26位点的染色质类似于野生型减数分裂细胞。这些观察结果表明，这些裂变酵母Tup 1样辅阻遏物抑制染色质重塑在CRE-related序列和Rst 2拮抗这一功能。
Chromatin remodeling plays crucial roles in the regulation of gene expression and recombination. Transcription of the fission yeastfbp1+gene and recombination at the meiotic recombination hotspotade6-M26(M26) are both regulated by cAMP responsive element (CRE)-like sequences and the CREB/ATF-type transcription factor Atf1•Pcr1. The Tup11 and Tup12 proteins, the fission yeast counterparts of theSaccharomyces cerevisiaeTup1 corepressor, are involved in glucose repression of thefbp1+transcription. We have analyzed roles of the Tup1-like corepressors in chromatin regulation around thefbp1+promoter and theM26hotspot. We found that the chromatin structure around two regulatory elements forfbp1+was remodeled under derepressed conditions in concert with the robust activation offbp1+transcription. Strains withtup11Δtup12Δ double deletions grown in repressed conditions exhibited the chromatin state associated with wild-type cells grown in derepressed conditions. Interestingly, deletion ofrst2+, encoding a transcription factor controlled by the cAMP-dependent kinase, alleviated thetup11Δtup12Δ defects in chromatin regulation but not in transcription repression. The chromatin at theM26site in mitotic cultures of atup11Δtup12Δ mutant resembled that of wild-type meiotic cells. These observations suggest that these fission yeast Tup1-like corepressors repress chromatin remodeling at CRE-related sequences and that Rst2 antagonizes this function.