Novel Plasmodium falciparum clones and rising clone multiplicities are associated with the increase in malaria morbidity in Ghanaian children during the transition into the high transmission season
Novel Plasmodium falciparum clones and rising clone multiplicities are associated with the increase in malaria morbidity in Ghanaian children during the transition into the high transmission season
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DOI：
10.1017/s0031182001008162
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发表时间：
2001-08-01
期刊：
影响因子：
2.4
通讯作者：
Arnot, DE
中科院分区：
文献类型：
--
作者：
Ofosu-Okyere, A;Mackinnon, MJ;Arnot, DE
对加纳儿童的恶性疟原虫感染和克隆多样性进行了调查，以研究疟疾传播季节开始对疾病发病率的影响。1998年2月至8月，每两周从居住在农村城镇Dodowa的40名儿童身上采集一次血样。计算恶性疟原虫寄生虫密度，并使用多态性MSP-1和MSP-2基因作为靶位点进行PCR基因分型，以估计每个样品中寄生虫克隆的数量。平均克隆数估计使用最大似然技术和最低数量的克隆每个病人进行了分析的影响，年龄，性别，季节，最低数量的克隆每个孩子，寄生虫病和寄生虫基因型的水平。统计分析表明，一个孩子携带的克隆越多，他们患疟疾的可能性就越大。在调整年龄和季节效应以及测量的循环寄生虫血症后，这是真实的。如果MSP-1 MAD 20和MSP-2 FC 27等位基因存在，则临床疾病的概率也增加。因此，这种纵向分析表明，加纳儿童出现有症状的疟疾发作的概率与恶性疟原虫克隆的数量和新类型的增加呈正相关。
A survey of Plasmodium falciparum infection and clone multiplicity in Ghanaian children was carried out to study the effect of the onset of the malaria transmission season on disease incidence. Fortnightly blood samples were collected from 40 children living in the rural town of Dodowa, between February and August 1998. P. falciparum parasite densities were calculated and PCR genotyping was carried out using the polymorphic MSP-1 and MSP-2 genes as target loci for the estimation of the number of parasite clones in each sample. The average clone number was estimated using maximum likelihood techniques and the minimum number of clones per patient was analysed for the effects of age, sex, season, minimum number of clones per child, level of parasitaemia and parasite genotype. The statistical analysis indicated that the more clones a child carried, the more likely they were to have a clinical malaria episode. This was true after adjusting for age and season effects and for the measured circulating parasitaemia. The probability of clinical disease also increased if the MSP-1 MAD 20 and the MSP-2 FC 27 alleles were present. This longitudinal analysis thus indicates that the probability of a Ghanaian child having a symptomatic malaria episode is positively associated with both increasing numbers and novel types of P. falciparum clones.