Analysis of the factors involved in the retinoic acid-induced differentiation of a retinoid-hypersensitive embryonal carcinoma cell mutant.
Analysis of the factors involved in the retinoic acid-induced differentiation of a retinoid-hypersensitive embryonal carcinoma cell mutant.
复制标题
视黄酸诱导的类视黄醇过敏胚胎癌细胞突变体分化相关因素的分析。
DOI：
10.1006/excr.1994.1030
复制
发表时间：
1994
影响因子：
3.7
通讯作者：
McCormick,PJ
中科院分区：
文献类型：
--
作者：
Dietrich,JM;McCormick,PJ
Retinoic acid (RA) has been known to play an important role in cellular growth and differentiation as well as in vertebrate development. Manyin vitrocell cultures also respond to RA by differentiating. Perhaps the most widely studied of these cultures are embryonal carcinoma (EC) cells. We have used an RA-hypersensitive EC cell mutant, created by retroviral insertion, to analyze the activity of the identifiable components in the RA response pathway. We have analyzed the mRNA expression patterns of the retinoic acid receptors (RARs) α, β, and γ, the retinoid X receptors (RXRs) α, β, and γ, and the cellular retinoic acid binding proteins (CRABPs) I and II. Our results indicate that CRABP I, RAR β, and RAR γ mRNAs are expressed differentially between parent and RA-hypersensitive mutant cells. All three messages are present at higher basal levels and at earlier times after RA addition in the mutant relative to parental cells. All other elements examined are equivalently expressed. Therefore analyses of the expression patterns of CRABPs, RARs, and RXRs in these RA-hypersensitive cells point to the probable importance of CRABP I, RAR β, and RAR γ in the RA induction pathway and also indicate that CRABP II and RXR γ are not likely to be critical elements in the early differentiative response of cells to RA.
