Increased helper inducer and decreased suppressor inducer phenotypes in the rheumatoid joint.
Increased helper inducer and decreased suppressor inducer phenotypes in the rheumatoid joint.
复制标题
类风湿关节中辅助诱导剂的增加和抑制诱导剂表型的减少。
DOI：
10.1002/art.1780310108
复制
发表时间：
1988
影响因子：
--
通讯作者：
Pope,RM
中科院分区：
文献类型：
--
作者：
Lasky,HP;Bauer,K;Pope,RM
从类风湿性关节炎（RA）患者的关节中分离的细胞表现出功能性免疫异常，例如抑制剂活性降低、对有丝分裂原的应答降低和免疫球蛋白产生增强。我们试图描述RA患者滑液（SF）和外周血（PB）中T淋巴细胞亚群的特征，试图阐明这些功能性免疫异常的机制。我们使用了双重免疫荧光染色技术，使用了几种单克隆抗体的组合，包括抗4 B4和抗2 H4，它们分别定义了CD 4+（Leu-3+和T4+）细胞的辅助诱导物和抑制诱导物亚群。在染色后，通过流式细胞术分析来自正常PB（n = 9）、RA PB（n = 6）和RA SF（n = 9）的单核细胞。RA SF中具有辅助诱导表型（CD 4+，4 B 4+）的细胞数量显著高于RA患者或正常对照PB中的水平（P <0.0002），而具有抑制诱导表型（CD 4+，2 H 4+）的细胞数量显著低于RA患者或正常对照PB中的水平（P<0.0002）。SF患者外周血CD_（8+）、~（2 H）~（4+）亚群明显低于PB患者（P< 0.0001）。RA患者外周血淋巴细胞亚群水平与正常人比较无显著性差异。这些观察结果可能部分解释了抑制活性降低、对有丝分裂原反应差、自体混合淋巴细胞反应以及类风湿关节中观察到的IG和类风湿因子产生增强的原因。
Cells isolated from the joints of patients with rheumatoid arthritis (RA) exhibit functional immune abnormalities, such as diminished suppressor activity, depressed response to mitogens, and enhanced immunoglobulin production. We sought to characterize the T lymphocyte subsets in the synovial fluid (SF) and peripheral blood (PB) of RA patients in an attempt to clarify the mechanism(s) responsible for these functional immune abnormalities. We used dual‐immunofluorescence staining techniques with several combinations of monoclonal antibodies, including anti‐4B4 and anti‐2H4, which define, respectively, the helper inducer and suppressor inducer subsets of CD4+ (Leu‐3+ and T4+) cells. Mononuclear cells from normal PB (n = 9), RA PB (n = 6), and RA SF (n = 9) were analyzed, after staining, by flow cytometry. We observed a significant increase (P< 0.0002) in the number of cells bearing the helper inducer phenotype (CD4+, 4B4+), and a significant decrease (P< 0.0002) in the number of cells bearing the suppressor inducer phenotype (CD4+, 2H4+), in RA SF compared with the levels in PB from RA patients or normal control subjects. We also observed that the CD8+, 2H4+ subset was significantly decreased (P< 0.0001) in SF compared with that in PB. There was no significant difference in the lymphocyte subset levels in PB from RA patients and from normal subjects. These observations may account, in part, for the reduced suppressor activity, the poor response to mitogens, and the autologous mixed lymphocyte reaction, as well as the enhanced production of Ig and rheumatoid factor, that are observed in the rheumatoid joint.