Methods to study protein dynamics and folding by mass spectrometry
Methods to study protein dynamics and folding by mass spectrometry
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DOI：
10.1016/j.ymeth.2004.03.015
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发表时间：
2004-09-01
期刊：
影响因子：
4.8
通讯作者：
Kaltashov, IA
中科院分区：
文献类型：
--
作者：
Eyles, SJ;Kaltashov, IA
很明显，仅仅知道蛋白质的结构不足以完全理解其行为：了解蛋白质内部发生的动态事件对于阐明其功能和折叠至关重要。近年来，质谱作为一种强大的生物物理方法已经走到了前列，它可以揭示蛋白质的结构和动力学。通过质谱监测的氢交换是溶液中骨架动力学的高度灵敏的标记物，其与气相解离技术相结合，可以提供高分辨率的工具来定位蛋白质的动态区域。此外，电喷雾质谱中的电荷态分布产生洞察力的性质和人口的交替结构状态存在于平衡。在本文中，我们描述了这些方法的几个应用程序来探测动态事件的关键蛋白质的结构，折叠和生物功能。(C)2004年爱思唯尔公司All rights reserved.
It is clear that merely knowing the structure of a protein alone is not sufficient to fully understand its behavior: knowledge also of the dynamic events that occur within proteins is vital to elucidate their function and folding. In recent years, mass spectrometry has come to the forefront as a powerful biophysical method, which can shed light both on the structure and dynamics of proteins. Hydrogen exchange monitored by mass spectrometry is a highly sensitive marker of the backbone dynamics in solution that, combined with gas phase dissociation techniques, can provide a high resolution tool to locate the dynamic regions of a protein. Additionally, charge state distributions in electrospray mass spectra yield insight into the nature and population of alternate structural states present at equilibrium. In this paper, we describe several applications of these methodologies to probe the dynamic events key to the structure, folding, and biological functions of proteins. (C) 2004 Elsevier Inc. All rights reserved.