Functional and molecular characteristics of T-cell growth factor.
Functional and molecular characteristics of T-cell growth factor.
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T 细胞生长因子的功能和分子特征。
DOI：
10.1016/0161-5890(80)90156-x
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发表时间：
1980
影响因子：
3.6
通讯作者：
Ruscetti,FW
中科院分区：
文献类型：
--
作者：
Smith,KA;Baker,PE;Gillis,S;Ruscetti,FW
t细胞生长因子是一种从凝集素或抗原激活的单核细胞中释放的有丝分裂蛋白，维持t淋巴细胞的连续指数增殖培养。探索TCGF产生所需参数的研究表明，成熟胸腺依赖性t细胞和贴壁细胞都参与TCGF的释放。进一步的实验表明，t细胞在接收两种信号后释放TCGF；(1)凝集素或抗原细胞膜结合，(2)从贴壁细胞中获得的可溶性产物。另一种不需要胸腺影响的t细胞亚群对TCGF有反应。TCGF似乎通过特定的膜受体与TCGF应答亚群相互作用：TCGF只能被凝集素或抗原激活的活t细胞或戊二醛固定t细胞吸收。t细胞反应由凝集素或抗原启动，但增殖仅由纯化的TCGF介导。因此，t细胞的免疫反应，在抗原反应细胞的克隆扩增和分化中达到高潮，似乎是由一种内源性衍生的增殖激素控制的，这是实现一个有能力的效应t细胞所必需的。
T-cell growth factor, a mitogenic protein released from lectin- or antigen-activated mononuclear cells, maintains T-lymphocytes in continuous exponential proliferative culture. Studies which explored the parameters required for TCGF production revealed that both mature thymic-dependent T-cells and adherent cells participated in the release of TCGF. Further experimentation suggested that T-cells released TCGF after receiving two signals; (1) lectin or antigen cell membrane binding, and (2) a soluble product derived from adherent cells. A separate subset of T-cells, which did not require thymic influence, responded to TCGF. TCGF appeared to interact with the TCGF-responsive subset via specific membrane receptors: TCGF could be absorbed only by lectin- or antigen-activated living or glutaraldehyde-fixed T-cells. The T-cell response was initiated by lectin or antigen, but proliferation was mediated solely by purified TCGF. Thus, the T-cell immune response, which culminates in clonal expansion and differentiation of antigenreactive cells, appears to be controlled by an endogenously-derived proliferative hormone, which is obligatory for the realization of a competent effector T-cell.