Polymorphic microsatellite markers for the symbiotic fungi cultivated by leaf cutter ants (Attini, Formicidae)

Polymorphic microsatellite markers for the symbiotic fungi cultivated by leaf cutter ants (Attini, Formicidae)
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切叶蚁(Attini,蚁科)培养的共生真菌的多态性微卫星标记

DOI:
10.1111/j.1755-0998.2009.02684.x
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发表时间:
2009
影响因子:
7.7
通讯作者:
U. Mueller
U. Mueller
中科院分区:
生物学1区
文献类型:
--
作者:
J. Scott;MATTHEW K. Weskin;Michael Cooper;U. Mueller

文献摘要

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我们为切叶蚁培育的共生真菌开发了23个多态微卫星标记,然后评估了北美切叶真菌种群(墨西哥、古巴、美国)的等位基因变异。23个基因座中有21个为多倍体,这与切叶真菌的多核特性一致。微卫星指纹技术现在可以评估切叶机巢内真菌的遗传变异,以测试栽培真菌的单一培养。
We developed 23 polymorphic microsatellite markers for the symbiotic fungi cultivated by leaf cutter ants, then assessed allelic variation in North American leafcutter‐fungus populations (Mexico, Cuba, USA). Polyploidy was indicated by 21 of the 23 loci, consistent with the multinucleate nature of leafcutter fungi. Microsatellite fingerprinting can now assess fungal genetic variation within leafcutter nests to test for monoculture of the cultivated fungi.