Somatic hybridization between Lycopersicon esculentum and Lycopersicon pennellii
Somatic hybridization between Lycopersicon esculentum and Lycopersicon pennellii
复制标题
番茄与番茄的体细胞杂交
DOI：
10.1007/bf00264476
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发表时间：
1985
影响因子：
5.4
通讯作者：
Maureen R. Hanson
中科院分区：
文献类型：
--
作者：
Mary A. O’Connell;Maureen R. Hanson
Selection and screening methods were devised which resulted in the identification of a number of somatic hybrid callus clones following fusion ofLycopersicon esculentumprotoplasts andL. pennelliisuspension culture protoplasts. Visual selection for callus morphology combined with a high fusion frequency and irradiation of one parental protoplast type (137Cs source, 1.5 Krads) resulted in selection of a callus clone population containing a high proportion of somatic hybrids. Analysis of a dimeric isozyme for the presence of a heterodimeric form was found to be satisfactory for distinguishing parental-type calli, somatic hybrid calli, and mixed calli derived from both types of unfused parental cells. No somatic hybrid calli produced shoots, although the sexual hybrid betweenL. esculentumandL. pennelliiregenerated well under the culture conditions employed. This result suggests that the non-regenerable growth habit of theL. pennelliisuspension culture was dominant in the somatic hybrid. The culture conditions described here are suitable for obtaining regenerated plants fromL. esculentummesophyll protoplasts.L. esculentumprotoplast calli from fusion cultures gave rise to shoots withL. esculentumphenotype at higher frequency than calli from control unfusedL. esculentummesophyll protoplast cultures. The use of probes for species-specific organelle DNA fragments allowed identification of organelle DNA restriction fragments in digests of total DNA from small samples of individual callus clones. The callus clones analyzed either carried predominantly one parental plastid DNA type or mixtures of both types. Use of a mitochondrial DNA (mtDNA) probe which distinguishes two parental mtDNA fragments revealed that theL. pennellii-specific fragment was present in all clones examined, but theL. esculentumfragment was absent or in low proportion.
