A close look at axonal transport: Cargos slow down when crossing stationary organelles.
A close look at axonal transport: Cargos slow down when crossing stationary organelles.
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DOI：
10.1016/j.neulet.2015.10.066
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发表时间：
2016-01-01
影响因子：
2.5
通讯作者：
Cui B
中科院分区：
文献类型：
--
作者：
Che DL;Chowdary PD;Cui B
货物沿细小轴突的双向运输是神经元结构、功能和存活的基础。轴突运输缺陷被认为与神经退行性疾病的机制有关。在本文中，我们研究了局部轴突环境对神经元中货物运输行为的影响。利用微流控神经元装置中的双色荧光成像，我们量化了货物穿过静止细胞器时的运输动力学，如静止的内小体和静止的轴突中的线粒体。我们发现轴突在静止细胞器附近有减慢或短暂停顿的趋势。TrkA、溶酶体和TrkB三种不同的货物在逆行和顺行运输方向都观察到了减速效应。我们的结果与假设一致，即庞大的轴突结构可能是轴突运输的立体障碍。然而，结果并不排除导致细胞器静止的细胞机制也是导致货物在相同位置移动延迟的原因。
The bidirectional transport of cargos along the thin axon is fundamental for the structure, function and survival of neurons. Defective axonal transport has been linked to the mechanism of neurodegenerative diseases. In this paper, we study the effect of the local axonal environment to cargo transport behavior in neurons. Using dual-color fluorescence imaging in microfluidic neuronal devices, we quantify the transport dynamics of cargos when crossing stationary organelles such as non-moving endosomes and stationary mitochondria in the axon. We show that the axonal cargos tend to slow down, or pause transiently within the vicinity of stationary organelles. The slow-down effect is observed in both retrograde and anterograde transport directions of three different cargos (TrkA, lysosomes and TrkB). Our results agree with the hypothesis that bulky axonal structures can pose as steric hindrance for axonal transport. However, the results do not rule out the possibility that cellular mechanisms causing stationary organelles are also responsible for the delay in moving cargos at the same locations.