A doublet-separated sensitivity-enhanced HSQC for the determination of scalar and dipolar one-bond J-couplings
A doublet-separated sensitivity-enhanced HSQC for the determination of scalar and dipolar one-bond J-couplings
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DOI：
10.1023/a:1008301415843
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发表时间：
1999-02-01
影响因子：
2.7
通讯作者：
Grzesiek, S
中科院分区：
文献类型：
--
作者：
Cordier, F;Dingley, AJ;Grzesiek, S
一个简单的，灵敏度增强的HSQC实验中描述的间接维分离的支持和低场分量到不同的子光谱。该序列类似于广义TROSY方案;然而，在检测期间使用X核的解耦。对该方法的弛豫效应、精度和灵敏度进行了详细的分析。该方法在二维水翻转（1）H-（15）N HSQC中得到证实，该方法测量了泛素和丙型肝炎蛋白酶的各向同性和定向样品中的（1）J（HN）分裂。计算结果与从传统的（1）H耦合HSQC得到的分裂非常一致。
A simple, sensitivity-enhanced HSQC experiment is described which separates the upheld and downfield components in the indirect dimension into different subspectra. The sequence is similar to the generalized TROSY scheme; however, decoupling of the X-nucleus is used during detection. A detailed analysis of relaxation effects, precision and sensitivity of the method is presented. The approach is demonstrated in a two-dimensional water flipback (1)H-(15)N HSQC which measures (1)J(HN) splittings in isotropic and oriented samples of ubiquitin and the hepatitis C protease. The results are in excellent agreement with splittings obtained from a conventional (1)H-coupled HSQC.