Biosynthesis of the thiamin-thiazole in eukaryotes: Identification of a thiazole tautomer intermediate
Biosynthesis of the thiamin-thiazole in eukaryotes: Identification of a thiazole tautomer intermediate
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DOI：
10.1021/ja802140a
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发表时间：
2008-08-27
影响因子：
15
通讯作者：
Begley, Tadhg P.
中科院分区：
文献类型：
--
作者：
Chatterjee, Abhishek;Schroeder, Frank C.;Begley, Tadhg P.
硫胺素噻唑在真核生物中的生物合成仍然没有完全了解。在这份报告中，晚期中间体，紧密结合的活性位点的酿酒酵母噻唑合酶，被确定为腺苷化噻唑互变异构体。评价了这种不寻常化合物的反应性。它的鉴定提供了由真核噻唑合酶催化的复杂反应序列的额外分子快照，并确定了硫胺素-噻唑生物合成的最后一步。
Thiamin thiazole biosynthesis in eukaryotes is still not completely understood. In this report, a late intermediate, tightly bound to the active site of the Saccharomyces cerevisiae thiazole synthase, was identified as an adenylated thiazole tautomer. The reactivity of this unusual compound was evaluated. Its identification provides an additional molecular snapshot of the complex reaction sequence catalyzed by the eukaryotic thiazole synthase and identifies the final step of the thiamin-thiazole biosynthesis.