Climatic factors and fertilization rates co-regulate anaerobic methane oxidation driven by multiple electron acceptors in Chinese paddy fields
Climatic factors and fertilization rates co-regulate anaerobic methane oxidation driven by multiple electron acceptors in Chinese paddy fields
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DOI：
10.1016/j.jclepro.2024.140600
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发表时间：
2024-01
影响因子：
11.1
通讯作者：
Wang‐ting Yang;Wei-qi Wang;E. Agathokleous;Yanan Bai;Shuai Zhang;Chun Wang;Yan-fang Feng;Jia-qi Liu;Yu-ling Yang;Caijun Geng;Li-dong Shen
中科院分区：
文献类型：
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作者：
Wang‐ting Yang;Wei-qi Wang;E. Agathokleous;Yanan Bai;Shuai Zhang;Chun Wang;Yan-fang Feng;Jia-qi Liu;Yu-ling Yang;Caijun Geng;Li-dong Shen
稻田是甲烷的重要人为储存库，其淹没管理为甲烷厌氧氧化提供了理想的栖息地。在此背景下，一个新的进化枝厌氧甲烷氧化（ANME）古菌，被称为ANME-2d，已被确定为能够催化厌氧甲烷氧化与硝酸盐和金属氧化物还原过程中的稻田。然而，我们的理解的机制，厌氧甲烷氧化及其在调节稻田甲烷排放的关键作用仍然有限。本研究通过13 C标记的稳定同位素示踪实验，在中国不同气候带的稻田中定量测定了硝酸盐和铁（III）驱动的厌氧甲烷氧化速率。此外，它还使用定量聚合酶链反应和高通量测序技术调查了ANME-2d古菌群落。硝酸盐驱动的甲烷厌氧氧化对甲烷减排的贡献率为10.9%。这一贡献等于先前确定的通过NC 10细菌介导的亚硝酸盐驱动的厌氧甲烷氧化的贡献（11.2%），但比铁驱动的贡献（4.1%）更重要。硝酸盐和亚硝酸盐驱动的甲烷厌氧氧化速率在不同气候带间差异显著，并与年平均温度呈正相关。此外，在代表性的浓度路径2.6和8.5下，它们的比率分别对高纬度和低纬度的温度升高更为敏感。硝态氮驱动的甲烷厌氧氧化速率与施氮量呈正相关，与施磷量呈负相关。相反，由铁驱动的厌氧甲烷氧化速率与氮肥或磷肥施用率没有显着相关性。这项研究强调了厌氧甲烷氧化在减缓全球变暖方面的巨大潜力，特别是在未来气候变化和氮负荷升高的条件下。这些发现强调了将厌氧甲烷氧化作为甲烷排放预测模型中的一个重要参数的必要性。
Paddy fields constitute a substantial anthropogenic reservoir of methane, with their inundation management fostering an ideal habitat for anaerobic methane oxidation. Within this context, a novel clade of anaerobic methanotrophic (ANME) archaea, known as ANME-2d, has been identified as capable of catalyzing anaerobic methane oxidation in conjunction with nitrate and metal oxide reduction processes within paddy fields. Nevertheless, our comprehension of the mechanisms governing anaerobic methane oxidation and its pivotal role in regulating methane emissions within rice paddies remains limited. This study quantified the rates of nitrate- and iron (III)-driven anaerobic methane oxidation through13C-labeled stable isotope tracing experiments in Chinese paddy fields spanning diverse climate zones. Additionally, it investigated the ANME-2d archaeal community using quantitative polymerase chain reaction and high-throughput sequencing techniques. The nitrate-driven anaerobic methane oxidation contributed 10.9% to methane emission reduction. This contribution is equal to the previously identified contribution of nitrite-driven anaerobic methane oxidation (11.2%) mediated via NC10 bacteria, but played more important roles than iron-driven one (4.1%). The rates of nitrate- and nitrite-driven anaerobic methane oxidation differed significantly among climate zones and showed positive correlation with the mean annual temperature. Furthermore, their rates were more sensitive to temperature increases at higher and lower latitudes, respectively, under both representative concentration pathways 2.6 and 8.5. The rate of anaerobic methane oxidation driven by nitrate exhibited a positive correlation with nitrogen fertilization rate but displayed a negative correlation with phosphorus fertilization rate. Conversely, the rate of anaerobic methane oxidation driven by iron showed no significant correlation with either nitrogen or phosphorus fertilization rates. This study underscores the great potential of anaerobic methane oxidation in mitigating global warming, particularly under the conditions of future climate change and elevated nitrogen loading. These findings underline the necessity of incorporating anaerobic methane oxidation as a crucial parameter in methane emission prediction models.