Roles of fibroblast growth factor 10 (Fgf10) in adipogenesis in vivo
Roles of fibroblast growth factor 10 (Fgf10) in adipogenesis in vivo
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DOI：
10.1016/j.mce.2003.12.017
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发表时间：
2004-04-15
影响因子：
4.1
通讯作者：
Itoh, N
中科院分区：
文献类型：
--
作者：
Asaki, T;Konishi, M;Itoh, N
Fgf10（-/-）小鼠胚胎的白色脂肪组织（WAT）发育严重受损。在这里，我们研究了Fgf10在体内脂肪形成中的作用机制。Fgf10（-/-）胚胎WAT细胞的增殖活性明显降低。我们还检测了转录因子C/EBPbeta， C/EBPalpha和PPARgamma的表达，这些转录因子对脂肪形成很重要。虽然Fgf10（-/-）胚胎WAT中C/EBPbeta和PPARgamma的表达量大大降低，但C/EBPalpha的表达量基本不变。因此，我们检测了它们在C/EBPalpha（-/-）胚胎WAT中的表达。虽然WAY中C/EBPbeta和PPARgamma的表达显著降低，但Fgf10的表达基本不变。由于这些在体内的结果似乎与体外报道的C/EBPbeta诱导PPARgamma和C/EBPalpha表达的转录级联模型相矛盾，我们还在不同发育阶段的野生型胚胎中检测了它们的表达。其次是Fgf10和C/ ebppha的表达，其次是C/EBPbeta和PPARgamma的表达。目前的研究结果表明，Fgf10而不是C/EBPalpha是前脂肪细胞增殖所必需的。相反，Fgf10和C/EBPalpha在不同的平行通路中协同作用是分化所必需的。出乎意料的是，这里描述的体内脂肪生成的转录级联与先前报道的体外级联不同。2004爱思唯尔爱尔兰有限公司版权所有。
The development of white adipose tissue (WAT) of Fgf10(-/-) mouse embryos was greatly impaired. Here, we examined the mechanism of Fgf10 action in adipogenesis in vivo. The proliferative activity in the WAT of Fgf10(-/-) embryos was greatly decreased. We also examined the expression of transcription factors, C/EBPbeta, C/EBPalpha and PPARgamma, that are important for adipogenesis. Although the expression of C/EBPbeta and PPARgamma in the WAT of Fgf10(-/-) embryos was greatly decreased, the expression of C/EBPalpha was essentially unchanged. Therefore, we examined their expression in the WAT of C/EBPalpha(-/-) embryos. Although the expression of C/EBPbeta and PPARgamma in the WAY was greatly decreased, the expression of Fgf10 was essentially unchanged. As these results in vivo appeared to be contradictory to a transcriptional cascade model in vitro that C/EBPbeta induces the expression of PPARgamma and C/EBPalpha reported, we also examined their expression in the WAY of wild type embryos at different developmental stages. The expression of Fgf10 and C/EBPalpha was followed by that of C/EBPbeta and PPARgamma. The present findings indicate that Fgf10 but not C/EBPalpha is required for the proliferation of preadipocytes. In contrast, both Fgf10 and C/EBPalpha acting synergistically in separate, parallel pathways are required for the differentiation. Unexpectedly, the transcriptional cascade of adipogenesis in vivo described here is distinct from the cascade in vitro previously reported. (C) 2004 Elsevier Ireland Ltd. All rights reserved.