An improved method for the determination of human erythrocyte pyrimidine 5'-nucleotidase activity.
An improved method for the determination of human erythrocyte pyrimidine 5'-nucleotidase activity.
复制标题
一种测定人红细胞嘧啶5-核苷酸酶活性的改进方法。
DOI：
10.1016/0009-8981(78)90155-9
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
M. Weyden
中科院分区：
文献类型：
--
作者：
P. H. Ellims;Lorraine Bailey;M. Weyden
本文介绍了一种测定人外周血溶血液中嘧啶5′-核苷酸酶活性的放射性方法。底物[14 C]尿苷一磷酸转化为[14 C]尿苷。本文提出了两种分离反应产物的简便、快速的方法，即采用下行层析法和从DEAE纤维素纸上洗脱法。用本法测定人红细胞嘧啶5′-核苷酸酶的比活力为6.7 ± 2.7（± 1 S. D.）nmol/h/mg蛋白质，该活性似乎广泛分布于人体组织中。
A radiometric method is described for measuring pyrimidine 5′-nucleotidase activity in human peripheral blood haemolysate. The substrate [14C]uridine monophosphate is converted to [14C]uridine. Two simple and rapid methods to isolate the product of the reaction employing either descending chromatography or elution from DEAE cellulose paper have been developed. With the described methods the specific activity of pyrimidine 5′-nucleotidase of human erythrocytes is 6.7 ± 2.7 (± 1 S.D.) nmol/h/mg protein and this activity appears to be widely distributed in human tissue.