Age-related changes in reactive oxygen species production in rat brain homogenates
Age-related changes in reactive oxygen species production in rat brain homogenates
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DOI：
10.1016/s0892-0362(99)00069-0
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发表时间：
2000-03-01
影响因子：
2.9
通讯作者：
Mundy, WR
中科院分区：
文献类型：
--
作者：
Driver, AS;Kodavanti, PRS;Mundy, WR
活性氧（ROS）的产生和由此产生的氧化应激与年龄相关的神经退行性疾病或暴露于环境化学物质导致的脑功能障碍的机制有关。我们研究了在存在和不存在 Fe2+ 的情况下，不同大脑区域产生 ROS 的能力的内在年龄相关差异。成年大鼠粗脑匀浆中ROS的产生与时间和组织浓度呈线性关系，并且与TEARS的产生相比，Fe2+对ROS的刺激程度更大。然后使用荧光探针 2',7'-二氯二氢荧光素 (DCFH) 测定 7 日龄、14 日龄、21 日龄、成年（3-6 月龄）和老年（24 月龄）大鼠大脑皮层、纹状体、海马和小脑匀浆中 ROS 的产生。 7 日龄、14 日龄和 21 日龄大鼠的 ROS 产生基础水平相似，成年大鼠增加，老年大鼠最高，并且脑区域之间没有差异。 Fe2+ (0.3-30 μM) 以浓度依赖性方式刺激所有大脑区域中的 ROS 产生。然而，Fe2+ 对 ROS 产生的刺激作用随年龄而变化。与成年和老年动物相比，14 日龄和 21 日龄大鼠的 ROS 产生量更高。与成年和老年大鼠相比，7 日龄大鼠的 ROS 产生在低 Fe2+ 浓度下减少，在高 Fe2+ 浓度下增加。这些数据表明，新生大鼠的大脑匀浆对 Fe2+ 的反应不同，并表明发育中的动物在接触有毒物质后可能对大脑中的氧化应激更加敏感。由爱思唯尔科学公司出版
The generation of reactive oxygen species (ROS) and resultant oxidative stress have been implicated in the mechanism of brain dys function due to age-related neurodegenerative diseases or exposure to environmental chemicals. We have investigated intrinsic age related differences in the ability of the various brain regions to generate ROS in the absence and presence of Fe2+. ROS production in crude brain homogenates from adult rats was linear with respect to time and tissue concentration, and was stimulated to a greater extent by Fe2+ than was TEARS production. ROS production was then determined in homogenates from cerebral cortex, striatum, hippocampus, and cerebellum of 7-day-old, 14-day-old, 21-day-old, adult (3-6-month old), and aged (24-month-old) rats using the fluorescent probe 2',7'-dichlorodihydrofluorescin (DCFH). Basal levels of ROS production were similar in 7-, 14-, and 21-day olds, increased in adults, and highest in aged rats, and did not differ between brain regions. ROS production was stimulated by Fe2+ (0.3-30 mu M) in a concentration-dependent manner in all brain regions. However, the stimulation of ROS production by Fe2+ varied with age. ROS production was greater in 14- and 21-day-old rats compared with adult and aged animals. ROS production in 7-day-old rats was decreased at low Fe2+ concentrations and increased at high Fe2+ concentrations compared to adult and aged rats. These data show that brain homogenates from neonatal rats respond differently to Fe2+, and suggest that developing animals may be more sensitive to oxidative stress in the brain after exposure to toxicants. Published by Elsevier Science Inc.