Megagametogenesis in Arabidopsis wild type and the Gf mutant
Megagametogenesis in Arabidopsis wild type and the Gf mutant
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DOI：
10.1007/s004970050067
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发表时间：
1997-02-01
影响因子：
--
通讯作者：
Drews, GN
中科院分区：
文献类型：
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作者：
Christensen, CA;King, EJ;Drews, GN
雌配子体是被子植物繁殖的重要结构，它介导许多繁殖功能，并在受精后产生大部分种子。在这里，我们描述了一种使用共焦激光扫描显微镜（CLSM）分析拟南芥雌配子体结构的快速方法。我们对野生型拟南芥中的大配子发生进行了全面的描述。根据我们的观察，我们将拟南芥大配子发生分为八个形态上不同的阶段。我们发现，助细胞退化是由授粉引发的，在四核阶段发生了剧烈的核迁移，并且雌蕊内的巨配子发生是相当同步的。最后，我们对先前报道的 Gf 突变体 (Redei 1965) 进行了表型分析，并表明它影响巨代谢发生的早期步骤。
The female gametophyte is an essential structure for angiosperm reproduction that mediates a host of reproductive functions and, following fertilization, gives rise to most of the seed. Here, we describe a rapid method to analyze Arabidopsis female gametophyte structure using confocal laser scanning microscopy (CLSM). We present a comprehensive description of megagametogenesis in wild-type Arabidopsis. Based on our observations, we divided Arabidopsis megagametogenesis into eight morphologically distinct stages. We show that synergid cell degeneration is triggered by pollination, that dramatic nuclear migrations take place during the four-nucleate stage, and that megagametogenesis within a pistil is fairly synchronous. Finally, we present a phenotypic analysis of the previously reported Gf mutant (Redei 1965) and show that it affects an early step of megaga-metogenesis.