Evidence of multiple complex patterns of T-DNA integration into the rice genome
Evidence of multiple complex patterns of T-DNA integration into the rice genome
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DOI：
10.1007/s001220050060
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发表时间：
2000-02
影响因子：
5.4
通讯作者：
Zhongchao Yin;Guo-Liang Wang
中科院分区：
文献类型：
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作者：
Zhongchao Yin;Guo-Liang Wang
从分子和遗传水平研究了从大肠杆菌中提取的双质粒长T-DNA （T-DNA和非T-DNA）转移到水稻基因组中的情况。在226个独立的转基因植物中，33%的转化体含有非t - dna序列。非t - dna转移的频率在三种革瘤杆菌之间没有显著差异。4个T1植株含有一个假定的长T-DNA插入，选择用于Southern分析。其中3个被证实有一个长度大于二元质粒单位长度的T-DNA插入。从这些植物中提取完整的双质粒进一步证实了这一点。我们的研究结果表明，通过转圈复制从大肠杆菌到水稻的长T-DNA转移是经常发生的，在生产商业化转基因水稻时应考虑到非T-DNA转移的高频率。
The transfer of the long T-DNA (T-DNA and non-T-DNA) of a binary plasmid fromAgrobacteriuminto the rice genome was investigated at both molecular and genetic levels. Out of 226 independent transgenic plants, 33% of the transformants contained non-T-DNA sequences. There was no major difference in the frequency of non-T-DNA transfer among threeAgrobacterium tumefaciensstrains.Four T1 plants containing a single putative long T-DNA insertion were selected for Southern analysis. Three of them were confirmed to have a long T-DNA insertion with a size of greater-than-unit-length of the binary plasmid. This was further confirmed by rescuing the intact binary plasmid from these plants. Our results suggest that long T-DNA transfer by rolling-circle replication fromAgrobacteriumto rice occurs frequently, and that the high frequency of non-T-DNA transfer should be considered when producing transgenic rice for commercial production.