Inability of natural killer cells to destroy autologous HIV-infected T lymphocytes
Inability of natural killer cells to destroy autologous HIV-infected T lymphocytes
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DOI：
10.1097/00002030-200303070-00003
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发表时间：
2003-03-07
期刊：
影响因子：
3.8
通讯作者：
Barker, E
中科院分区：
文献类型：
--
作者：
Bonaparte, MI;Barker, E
目的：确定自然杀伤（NK）细胞是否能够杀死HIV感染的自体原代T细胞blasts.Design：NK细胞杀死HIV感染的原代T细胞blasts，其细胞表面主要组织相容性复合物（MHC）I类分子减少的能力，在裂解assay.Methods：植物血凝素处理的CD 4 + T细胞在体外感染HIV-1。通过去除CD 4 + T细胞将感染的细胞与未感染的细胞分离。采用免疫磁珠分离法从同一供者外周血单个核细胞（PBMC）中分离NK细胞作为CD 4 + T细胞。从PBMC中分离的NK细胞，然后作为效应细胞和HIV感染的T-细胞母细胞作为靶细胞在裂解assay.Results：结果表明，HIV感染的主要CD 4 + T细胞的结果在61-68%的减少表面表达的MHC I类分子。尽管MHC I类表达降低，但NK细胞不能裂解自体HIV感染的T细胞母细胞，但在NK细胞敏感细胞系K562的裂解中是有效的。NK细胞不能裂解HIV感染的T细胞母细胞并不依赖于用于感染CD 4 + T细胞的HIV毒株。结论：这些研究表明，尽管HIV感染的T细胞母细胞的MHC I类分子表达急剧下降，但它们可以逃避自体NK细胞的破坏。(C)2003年利平科特威廉姆斯威尔金斯。
Objective: Determine whether natural killer (NK) cells are capable of killing HIV-infected autologous primary T-cell blasts.Design: The ability of NK cells to kill HIV-infected primary T-cell blasts, whose cell surface major histocompatibility complex (MHC) class I molecules was decreased, was evaluated in a lytic assay.Methods: Phytohemagglutinin-treated CD4+ T cells were infected in vitro with HIV-1. Infected cells were separated from uninfected cells by removal of CD4+ T cells. The NK cells were isolated from peripheral blood mononuclear cells (PBMC) of the same donor as the CD4+ T cells by immunomagnetic bead separation. The NK cells isolated from PBMC were then used as effector cells and the HIV-infected T-cell blasts were used as target cells in a lytic assay.Results: It was demonstrated that HIV infection of primary CD4+ T cells results in a 61-68% reduction in surface expression of MHC class I molecules. Despite the decreased MHC class I expression the NK cells were incapable of lysing autologous HIV-infected T-cell blasts, yet were effective in the lysis of the NK cell sensitive cell line, K562. The inability of NK cells to lyse HIV-infected T-cell blasts is not dependent on the strain of HIV used to infected the CD4+ T cellConclusion: These studies indicate that despite drastic decreases in MHC class I molecule expression, HIV-infected T-cell blasts can evade destruction by autologous NK cells. (C) 2003 Lippincott Williams Wilkins.