The retention index approach in liquid chromatography: An historical review and recent advances
The retention index approach in liquid chromatography: An historical review and recent advances
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DOI：
10.1016/j.chroma.2021.461963
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发表时间：
2021-02-18
影响因子：
4.1
通讯作者：
Mondello, Luigi
中科院分区：
文献类型：
--
作者：
Rigano, Francesca;Arigo, Adriana;Mondello, Luigi
历史上，保留参数被可靠地用作色谱分析系统中的鉴别标准。然而，纯分析标准品是必要的，以确定给定化合物的保留行为。近几十年来，质谱仪（MS）成为获得未知峰结构信息的首选检测器，这要归功于碎片的解析，这些碎片通常是由于特定官能团的丢失而产生的。与使用保留数据相比，操作员的成本和经验水平肯定更高。因此，本综述的目的是描述在常规的、实验室间适用的鉴别程序中引入线性保留指数（LRI）的努力。将讨论的要求和主要挑战，甚至相比，气相色谱法，其中LRI是稳定地用于识别目的，通常与MS光谱库相结合。大量的LC-顺从分子和广泛的LC移动的相组成使得通用LRI数据库的建立是一项非常具有挑战性的任务。报告了过去几十年中遇到的限制，以及为克服这些问题提出的新建议。(c)2021爱思唯尔有限公司版权所有。
Historically, retention parameters were reliably used as identification criterion in chromatographic analytical systems. However, pure analytical standards are necessary to determine the retention behaviour of a given compound. In recent decades, mass spectrometer (MS) became the detector of choice to obtain structural information on unknown peaks, thanks to the elucidation of fragments, often arisen from the loss of specific functional groups. The cost and the level of experience of the operators is surely higher compared to the use of retention data. Therefore, the aim of the present review is to describe the effort s in the introduction of the Linear Retention Index (LRI) in routine, interlaboratory applicable identification procedures. The requirements and the main challenges will be discussed, even compared to gas chromatography methods, in which LRI is stably used for identification purposes, usually in combination with MS spectral libraries. The higher number of LC-amenable molecules and the wide range of LC mobile phase compositions make the building of universal LRI database a very challenging task. The limitations encountered in the past decades are reported, together with new proposals in order to overcome such issues. (c) 2021 Elsevier B.V. All rights reserved.