Minocycline inhibits antigen processing for presentation to human T cells: additive inhibition with chloroquine at therapeutic concentrations
Minocycline inhibits antigen processing for presentation to human T cells: additive inhibition with chloroquine at therapeutic concentrations
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DOI：
10.1016/j.clim.2004.07.012
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发表时间：
2004-12-01
影响因子：
8.6
通讯作者：
Koujak, S
中科院分区：
文献类型：
--
作者：
Kalish, RS;Koujak, S
二甲胺四环素抑制破伤风类毒素（TT）向人T细胞呈递的能力进行了测试。外周血抗原呈递细胞（APC）在加入测试化合物前后用TT孵育4小时，然后用多聚甲醛固定APC，并加入自体TT应答T细胞系进行增殖试验。二甲胺四环素（0.1-0.4 mM）对TT有明显的抑制作用，与氯喹相当。在米诺环素之前，TT与APC孵育未观察到抑制作用，表明预处理抗原的呈递未被抑制。二甲胺四环素、强力霉素和四环素均能抑制PBMC对TT的增殖。二甲胺四环素和氯喹联用导致两种药物的加性抑制达到临床相关水平（3.7 muM）。本研究提示二甲胺四环素具有新的免疫抑制机制，并可能与氯喹或羟氯喹联用时具有附加抗炎作用。(C) 2004爱思唯尔公司版权所有。
The ability of minocycline to inhibit processing of tetanus toxoid (TT) for presentation to human T cells was tested. Peripheral blood antigen presenting cells (APC) were incubated with TT before or after addition of test compounds for 4 h. APC were then fixed with paraformaldehyde, and added to autologous TT-responsive T cell lines for a proliferation assay. Minocycline (0.1-0.4 mM) gave significant inhibition of T cell response to TT and was equivalent to chloroquine. Inhibition was not observed when TT was incubated with APC before minocycline, indicating that presentation of preprocessed antigen was not inhibited. Minocycline, doxycycline, and tetracycline all inhibited the proliferation of PBMC to TT. The combination of minocycline and chloroquine resulted in additive inhibition at clinically relevant levels of both drugs (3.7 muM). This study suggests a novel immunosuppressive mechanism for minocycline, as well as possible additive anti-inflammatory effect when combined with chloroquine or hydroxychloroquine. (C) 2004 Elsevier Inc. All rights reserved.