Skeletal muscle neuronal nitric oxide synthase μ protein is reduced in people with impaired glucose homeostasis and is not normalized by exercise training
Skeletal muscle neuronal nitric oxide synthase μ protein is reduced in people with impaired glucose homeostasis and is not normalized by exercise training
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DOI：
10.1016/j.metabol.2007.06.003
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发表时间：
2007-10-01
影响因子：
9.8
通讯作者：
McConell, Glenn K.
中科院分区：
文献类型：
--
作者：
Bradley, Scott J.;Kingwell, Bronwyn A.;McConell, Glenn K.
2型糖尿病患者骨骼肌诱导型一氧化氮合酶（NOS）蛋白水平显著升高，而内皮NOS水平正常。糖尿病大鼠研究表明，骨骼肌神经元NOS（nNOS）μ蛋白表达可能在人胰岛素抵抗中减少。本研究的目的是确定骨骼肌nNOS μ蛋白的表达是否减少葡萄糖稳态受损的人，以及运动训练是否增加这些人的nNOS μ蛋白的表达，因为运动训练增加大鼠骨骼肌nNOS μ蛋白。7名2型糖尿病或前驱糖尿病（空腹血糖受损和/或糖耐量受损）患者和7名年龄在36至60岁之间的匹配（性别、年龄、健身、体重指数、血压、血脂）健康对照者参与了这项研究。在1周和4周的自行车运动训练（60% V-O2峰值，50分钟× 5 d wk（-1））之前和之后，获得用于nNOS mu蛋白测定的股外侧肌活检，测量有氧适应性（峰值肺氧摄取[V-O2峰值]），并评估葡萄糖耐量和胰岛素稳态。2型糖尿病或糖尿病前期患者的骨骼肌nNOS μ蛋白显著低于对照组（32%）（17.7 ± 1.2 vs 26.2 ± 3.4任意单位，P
Skeletal muscle inducible nitric oxide synthase (NOS) protein is greatly elevated in people with type 2 diabetes mellitus, whereas endothelial NOS is at normal levels. Diabetic rat studies suggest that skeletal muscle neuronal NOS (nNOS) mu protein expression may be reduced in human insulin resistance. The aim of this study was to determine whether skeletal muscle nNOS mu protein expression is reduced in people with impaired glucose homeostasis and whether exercise training increases nNOS mu protein expression in these individuals because exercise training increases skeletal muscle nNOS mu protein in rats. Seven people with type 2 diabetes mellitus or prediabetes (impaired fasting glucose and/or impaired glucose tolerance) and 7 matched (sex, age, fitness, body mass index, blood pressure, lipid profile) healthy controls aged 36 to 60 years participated in this study. Vastus lateralis muscle biopsies for nNOS mu protein determination were obtained, aerobic fitness was measured (peak pulmonary oxygen uptake [V-O2 peak]), and glucose tolerance and insulin homeostasis were assessed before and after 1 and 4 weeks of cycling exercise training (60% V-O2 peak, 50 minutes x 5 d wk(-1)). Skeletal muscle nNOS mu protein was significantly lower (by 32%) in subjects with type 2 diabetes mellitus or prediabetes compared with that in controls before training (17.7 +/- 1.2 vs 26.2 +/- 3.4 arbitrary units, P